Nicalin antibody (AA 401-500)

Catalog No. ABIN1386554

Product Details anti-Nicalin Antibody

Target: Nicalin (NCLN)

Binding Specificity: AA 401-500

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nicalin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Nicalin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Nicalin

Target: Nicalin (NCLN)

Alternative Name: Nicalin/NCLN (NCLN Products)

Synonyms: NET59 antibody, RGD1309355 antibody, wu:fb07e09 antibody, wu:fk52a06 antibody, zgc:77236 antibody, 3100002P13Rik antibody, AW120537 antibody, nicalin antibody, NCLN antibody, Bm1_29425 antibody, LOC100280679 antibody, Ncln antibody, ncln antibody

Background:

Synonyms: NCLN, NCLN_HUMAN, NET59, Nicalin, Nicalin homolog zebrafish, Nicastrin like protein, Nicastrin-like protein.

Background: Nicastrin is an integral part of the Alzheimers associated gamma-secretase complex. Nicalin, a nicastrin-like protein, is not associated with gamma-secretase but rather interacts with NOMO (Nodal modulator). Nodals are TGF beta signaling factors that control various cell fate decisions during embryonic body planning stages in vertebrate development. The Nicalin/NOMO complex acts to regulate the nodal signaling factors during gastrulation. This regulation most often affects the development of the axial mesoderm. Nodal signaling is an important factor for melanoma cell invasiveness and tumorigenicity and inhibition of this signal can promote melanoma cells reverting back toward a melanocyte phenotype.