CRIPT antibody (AA 21-80)

Catalog No. ABIN1386373

Product Details anti-CRIPT Antibody

Target: CRIPT (Cysteine-Rich PDZ-Binding Protein (CRIPT))

Binding Specificity: AA 21-80

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRIPT antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Predicted Reactivity: Human,Mouse,Rat,Cow,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CRIPT

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CRIPT

Target: CRIPT (Cysteine-Rich PDZ-Binding Protein (CRIPT))

Alternative Name: CRIPT (CRIPT Products)

Background:

Synonyms: CRIPT, CRIPT_HUMAN, Cysteine rich interactor of PDZ3, Cysteine rich PDZ binding protein, Cysteine-rich interactor of PDZ three, Cysteine-rich interactor of PDZ3, Cysteine-rich PDZ-binding protein, HSPC139, HSPC139 protein, Hypothetical protein CRIPT, Postsynaptic protein CRIPT.

Background: The PSD-95/SAP 90 family of proteins, which are known to bind to and cluster various membrane proteins, are involved in the organization of synaptic structure. These proteins are physically and functionally linked to cytoskeletal and/or signaling proteins. CRIPT (for cysteine-rich interactor of PDZ three), a novel postsynaptic protein, binds specifically to the PDZ3 domain of PSD-95/SAP 90. CRIPT induces the recruitment of PSD-95/SAP 90 to microtubules, and it has been shown to bind directly to microtubules, indicating that it may be responsible for cytoskeletal anchoring of PSD-95/SAP 90. CRIPT is widely expressed outside of the brain and is highly conserved from animals to plants suggesting a wider role in regulating cytoskeleton-membrane associations.