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LAT antibody (pTyr161)

Name: LAT antibody (pTyr161)
SKU: ABIN1386362
Price: 384.62 USD
Availability: InStock

LAT Reactivity: Human ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Phospho-LAT (Tyr161) Polyclonal Antibody, Unconjugated (ABIN1386362) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (LAT antibody (pTyr161))

IHC

Paraformaldehyde-fixed, paraffin embedded rat spleen tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-LAT (Tyr161) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (LAT antibody (pTyr161))

IHC (p)

2 images

Catalog No. ABIN1386362

Datasheet as PDF

Target See all LAT Antibodies

LAT (Linker For Activation of T Cells (LAT))
Binding Specificity
20

15

14

9

8

4

4

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pTyr161
Reactivity
114

46

29

1
Human
Host
106

9

2

1
Rabbit
Compatible Secondaries
Clonality
108

10
Polyclonal
Conjugate
67

4

4

3

3

3

3

3

3

3

3

3

3

3

3

1

1

1

1

1

1

1
This LAT antibody is un-conjugated
Application
76

45

38

38

15

14

11

8

4

3

2

1

1
ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity

Human,Mouse,Rat,Cow

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic phosphopeptide derived from human LAT around the phosphorylation site of Tyr161

Isotype

IgG

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Application Notes

ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

LAT (Linker For Activation of T Cells (LAT))

Alternative Name

LAT (LAT Products)

Background

Synonyms: LAT1, pp36, Linker for activation of T-cells family member 1, 36 kDa phospho-tyrosine adapter protein, p36-38, LAT
Background: Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.

Gene ID

27040

UniProt

O43561

Pathways

TCR Signaling, Fc-epsilon Receptor Signaling Pathway