NLRP3 antibody (AA 15-120)

Catalog No. ABIN1386361

Product Details anti-NLRP3 Antibody

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Binding Specificity: AA 15-120

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NLRP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NLRP3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-NLRP3 antibody (ABIN1386361)

Li, Jiang, Ye, Zhou, Liu, Yang, Hao, Hu: "P2Y14 receptor has a critical role in acute gouty arthritis by regulating pyroptosis of macrophages." in: Cell death & disease, Vol. 11, Issue 5, pp. 394, (2021) (PubMed).

Yang, Cheng, Liu, Liu, Tan, Guan, Guo, Kuang: "Datura Metel L. Ameliorates Imiquimod-Induced Psoriasis-Like Dermatitis and Inhibits Inflammatory Cytokines Production through TLR7/8-MyD88-NF-κB-NLRP3 Inflammasome Pathway." in: Molecules (Basel, Switzerland), Vol. 24, Issue 11, (2019) (PubMed).

Zendedel, Mönnink, Hassanzadeh, Zaminy, Ansar, Habib, Slowik, Kipp, Beyer: "Estrogen Attenuates Local Inflammasome Expression and Activation after Spinal Cord Injury." in: Molecular neurobiology, (2017) (PubMed).

Yu, Liu, Sun, Wang, Wang, Zhao, Dai, Zhao, Zhang, Li, Han, Lu, Dong, Liu, Yu, Li: "Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway." in: Oncotarget, Vol. 8, Issue 23, pp. 37705-37716, (2017) (PubMed).

Zhang, Pan, Yang, Fu, Liu, Chi, Zhang, Hong, Ma, Yin: "H3 Relaxin Protects Against Myocardial Injury in Experimental Diabetic Cardiomyopathy by Inhibiting Myocardial Apoptosis, Fibrosis and Inflammation." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 43, Issue 4, pp. 1311-1324, (2017) (PubMed).

Fernandez, Miller, Krammer, Gopal, Greenbaum, Bhardwaj: "Ion efflux and influenza infection trigger NLRP3 inflammasome signaling in human dendritic cells." in: Journal of leukocyte biology, Vol. 99, Issue 5, pp. 723-34, (2016) (PubMed).

Zhou, Rivera, Bhosle, Lahaie, Shao, Tahiri, Zhu, Polosa, Dorfman, Beaudry-Richard, Costantino, Lodygensky, Lachapelle, Chemtob: "Choroidal Involution Is Associated with a Progressive Degeneration of the Outer Retinal Function in a Model of Retinopathy of Prematurity: Early Role for IL-1β." in: The American journal of pathology, Vol. 186, Issue 12, pp. 3100-3116, (2016) (PubMed).

Debye, Schmülling, Zhou, Rune, Beyer, Johann: "Neurodegeneration and NLRP3 inflammasome expression in the anterior thalamus of SOD1(G93A) ALS mice." in: Brain pathology (Zurich, Switzerland), (2016) (PubMed).

Heitzer, Kaiser, Kanagaratnam, Zendedel, Hartmann, Beyer, Johann: "Administration of 17β-Estradiol Improves Motoneuron Survival and Down-regulates Inflammasome Activation in Male SOD1(G93A) ALS Mice." in: Molecular neurobiology, (2016) (PubMed).

Zendedel, Johann, Mehrabi, Joghataei, Hassanzadeh, Kipp, Beyer: "Activation and Regulation of NLRP3 Inflammasome by Intrathecal Application of SDF-1a in a Spinal Cord Injury Model." in: Molecular neurobiology, (2015) (PubMed).

Johann, Heitzer, Kanagaratnam, Goswami, Rizo, Weis, Troost, Beyer: "NLRP3 inflammasome is expressed by astrocytes in the SOD1 mouse model of ALS and in human sporadic ALS patients." in: Glia, (2015) (PubMed).

Target Details for NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Alternative Name: CIAS1 (NLRP3 Products)

Background:

Synonyms: NACHT, LRR and PYD domains-containing protein 3, Angiotensin/vasopressin receptor AII/AVP-like, Caterpiller protein 1.1, CLR1.1, Cold autoinflammatory syndrome 1 protein, Cryopyrin, PYRIN-containing APAF1-like protein 1, NLRP3, C1orf7, CIAS1, NALP3, PYPAF1

Background: May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.

Gene ID: 114548

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome