JARID2 antibody (AA 301-400)

Catalog No. ABIN1386206

Product Details anti-JARID2 Antibody

Target: JARID2 (Jumonji, AT Rich Interactive Domain 2 (JARID2))

Binding Specificity: AA 301-400

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JARID2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Jarid2/Jumonji

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for JARID2

Target: JARID2 (Jumonji, AT Rich Interactive Domain 2 (JARID2))

Alternative Name: Jarid2/Jumonji (JARID2 Products)

Synonyms: JMJ antibody, Jmj antibody, jumonji antibody, JARID2 antibody, jarid2 antibody, jmj antibody, si:dkey-211c3.1 antibody, Jumonji antibody, jarid2-a antibody, jarid2-b antibody, jarid2.S antibody, jarid2b antibody, jumonji and AT-rich interaction domain containing 2 antibody, jumonji, AT rich interactive domain 2 antibody, jumonji, AT rich interactive domain 2a antibody, jumonji, AT rich interactive domain 2b antibody, jumonji and AT-rich interaction domain containing 2 L homeolog antibody, JARID2 antibody, Jarid2 antibody, jarid2a antibody, jarid2 antibody, jarid2b antibody, jarid2.L antibody

Background:

Synonyms: JMJ, Protein Jumonji, Jumonji/ARID domain-containing protein 2, JARID2

Background: Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling.

Gene ID: 3720

UniProt: Q92833

Pathways: Chromatin Binding