SPPL2B antibody (AA 55-135)

Catalog No. ABIN1386177

Product Details anti-SPPL2B Antibody

Target: SPPL2B (Signal Peptide Peptidase-Like 2B (SPPL2B))

Binding Specificity: AA 55-135

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPPL2B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SPPL2B

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SPPL2B

Target: SPPL2B (Signal Peptide Peptidase-Like 2B (SPPL2B))

Alternative Name: IMP4/SPPL2B (SPPL2B Products)

Synonyms: SPPL2B antibody, wu:fc16e01 antibody, wu:fc85d12 antibody, zgc:136525 antibody, MGC147524 antibody, IMP-4 antibody, IMP4 antibody, PSH4 antibody, PSL1 antibody, 3110056O03Rik antibody, AW550292 antibody, RGD1308556 antibody, signal peptide peptidase-like 2 antibody, signal peptide peptidase like 2B antibody, signal peptide peptidase like 2B S homeolog antibody, signal peptide peptidase-like 2B antibody, sppl2 antibody, sppl2b antibody, sppl2b.S antibody, SPPL2B antibody, Sppl2b antibody

Background:

Synonyms: IMP4, Signal peptide peptidase-like 2B, Intramembrane protease 4, Protein SPP-like 2B, SPPL2B, SPP2B_HUMAN.

Background: SPPL2b is a 592 amino acid multi-pass membrane protein that contains one protease associated domain and belongs to the GXGD family of aspartic proteases. Localized to the membrane of the Golgi apparatus and to lysosomes, endosomes and the plasma membrane, SPPL2b functions as an intramembrane protease that specifically cleaves the transmembrane domain of TNF? Once cleaved, TNF?releases its intracellular domain, thus triggering the immunity-related expression of cytokines (also known as interleukins) which are used in various pathways throughout the body. SPPL2b also interacts with and catalyzes the intramembrane proteolysis of ITM2B (integral membrane protein 2B), a protein that, when mutated, is associated with dementia. Four isoforms of SPPL2b exist due to alternative splicing events.