POLB antibody (AA 55-160)

Catalog No. ABIN1386139

Product Details anti-POLB Antibody

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Binding Specificity: AA 55-160

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DNA Polymerase beta

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for POLB

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Alternative Name: DNA Polymerase beta (POLB Products)

Synonyms: wu:fa97a05 antibody, zgc:109924 antibody, POLB antibody, DDBDRAFT_0186152 antibody, DDBDRAFT_0215784 antibody, DDBDRAFT_0232376 antibody, DDB_0186152 antibody, DDB_0215784 antibody, DDB_0232376 antibody, A430088C08Rik antibody, polymerase (DNA directed), beta antibody, DNA polymerase beta antibody, DNA polymerase X family protein antibody, polymerase (DNA directed), beta S homeolog antibody, polb antibody, POLB antibody, polB antibody, polb.S antibody, Polb antibody

Background:

Synonyms: DNA polymerase beta, POLB

Background: Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases.

Gene ID: 5423

UniProt: P06746

Pathways: DNA Damage Repair