IKK-alpha /IKK-beta antibody (AA 151-250)

Catalog No. ABIN1386118

Product Details anti-IKK-alpha /IKK-beta Antibody

Target: IKK-alpha /IKK-beta

Binding Specificity: AA 151-250

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKK-alpha /IKK-beta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human IKK Alpha/IKK beta

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for IKK-alpha /IKK-beta

Target: IKK-alpha /IKK-beta

Alternative Name: IKK alpha / beta (IKK-alpha /IKK-beta Products)

Background:

Synonyms: IKK-Alpha, I kappa A kinase 2, I kappa A kinase Alpha, IkBKA, IKK 1, IKKA, IKK Alpha, IKK A, IKK Alpha, IKK1, IKKA, IKK-_, I kappa B kinase 2, I kappa B kinase beta, IkBKB, IKK 2, IKK_, IKK _, IKK B, IKK beta, IKK2, IKKB, Inhibitor of kappa light chain gene enhancer in B cells, Inhibitor of kappa light polypeptide gene enhancer in B cells, Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta, Inhibitor of nuclear factor kappa B kinase beta subunit, Inhibitor of nuclear factor kappa B kinase subunit beta, MGC131801, NFKBIKB, Nuclear factor NF kappa B inhibitor kinase beta, Nuclear factor of kappa light chain gene enhancer in B cells inhibitor.

Background: IKK Alpha/IKK beta is a member of the IKK complex which is composed of IKK alpha, IKK beta, IKK gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK beta has been shown to activate NF-kB and phosphorylate IKB alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKK beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK beta activity can also be regulated by MEKK1.