LIM Domain Binding 1 Protein antibody (AA 10-100)

Catalog No. ABIN1386034

Product Details anti-LIM Domain Binding 1 Protein Antibody

Target: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Binding Specificity: AA 10-100

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIM Domain Binding 1 Protein antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Dog,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human LDB1/CLIM-2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for LIM Domain Binding 1 Protein

Target: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Alternative Name: LDB1/CLIM-2 (LDB1 Products)

Synonyms: CLIM2 antibody, NLI antibody, chi antibody, clim2 antibody, nli antibody, xldb1 antibody, ldb1 antibody, LDB1 antibody, ldb4 antibody, ldb4b antibody, LIM domain binding 1 antibody, LIM domain binding 1 L homeolog antibody, LIM-domain binding 1b antibody, LIM domain binding protein 1 antibody, LIM domain binding 1a antibody, Ldb1 antibody, LDB1 antibody, ldb1.L antibody, ldb1b antibody, xldb1 antibody, ldb1a antibody

Background:

Synonyms: Carboxyl Terminal LIM Domain Binding 2, Carboxyl-terminal LIM domain-binding protein 2, CLIM 2, CLIM-2, hLdb1, LDB-1, ldb1, LDB1_HUMAN, LIM Domain Binding 1, LIM domain binding factor CLIM2, LIM domain-binding factor CLIM2, LIM domain-binding protein 1, NLI, Nuclear LIM Domain Interactor, Nuclear LIM interactor antibodyxldb1.

Background: The LIM-only (LMO) proteins, LMO1 and LMO2, are nuclear factors that are characterized by a conserved LIM domain. The LIM domain consists of a cysteine-rich zinc-binding motif that is present in a variety of transcription factors, including the LIM homeobox (LHX) proteins expressed in the central nervous system and involved in cell differentiation. LMO1 and LMO2 are expressed in the adult CNS in a cell type-specific manner, where they are differentially regulated by neuronal activity and are involved in regulating the cellular differentiated phenotype of neurons. LMO2 lacks a specific DNA-binding homeobox domain but rather assembles into transcriptional regulatory complexes to mediate gene expression by interacting with the widely expressed nuclear LIM interactor (NLI). NLI, known also as CLIM-1, and the related protein CLIM-2, facilitate the formation of heteromeric LIM complexes and also enhance the nuclear retention of LIM proteins. LMO2 and the related protein LMO4 are expressed in thymic precursor cells. LMO4 is also expressed in mature T cells, cranial neural crest cells, somite, dorsal limb bud mesenchyme, motor neurons, and Schwann cell progenitors.

Pathways: Stem Cell Maintenance, Chromatin Binding