FAD Synthetase antibody (AA 345-420)

Catalog No. ABIN1386004

Product Details anti-FAD Synthetase Antibody

Target: FAD Synthetase

Binding Specificity: AA 345-420

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAD Synthetase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Zebrafish

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FLAD1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for FAD Synthetase

Target: FAD Synthetase

Alternative Name: FLAD1 (FAD Synthetase Products)

Background:

Synonyms: FAD pyrophosphorylase, FAD synthetase, FAD synthetase region, FAD1, FAD1 flavin adenine dinucleotide synthetase homolog S. cerevisiae, Fad1, flavin adenine dinucleotide synthetase, homolog, Fad1, flavin adenine dinucleotide synthetase, homolog yeast, FADS, Flad1 protein, Flavin adenine dinucleotide synthetase, FMN adenylyl transferase, FMN adenylyltransferase, MGC187714, MGC31803, MGC40255, Molybdenum cofactor biosynthesis protein-like region, Pp591, RFad1, flavin adenine dinucleotide synthetase, homolog yeast.

Background: FLAD1 is a 587 amino acid protein where its N-terminus belongs to the moaB/mog family and its C-terminus belongs to the PAPS reductase family. Existing as five alternatively spliced isoforms, FLAD1 localizes to the cytoplasm and utilizes magnesium as a cofactor. FLAD1 is a key enzyme in the metabolic pathway that converts riboflavin into the redox cofactor flavin adenine dinucleotide (FAD). It is suggested that the molybdenum cofactor biosynthesis protein-like region of FLAD1 may not be functional.