UBE2M antibody (AA 1-100)

Catalog No. ABIN1385999

Product Details anti-UBE2M Antibody

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Binding Specificity: AA 1-100

Reactivity: Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2M antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Xenopus laevis

Predicted Reactivity: Human,Mouse,Rat,Cow,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human UBE2M

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for UBE2M

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Alternative Name: UBE2M (ube2m Products)

Synonyms: DDBDRAFT_0204633 antibody, DDBDRAFT_0238042 antibody, DDB_0204633 antibody, DDB_0238042 antibody, UBC-RS2 antibody, UBC12 antibody, hUbc12 antibody, 2510040H03Rik antibody, Ubc-rs2 antibody, ubiquitin conjugating enzyme E2M antibody, ubiquitin-conjugating enzyme E2M antibody, ubiquitin conjugating enzyme E2 M antibody, NEDD8-conjugating enzyme antibody, ubiquitin conjugating enzyme E2M S homeolog antibody, ube2m antibody, LOC692934 antibody, UBE2M antibody, Ube2m antibody, ube2m.S antibody

Background:

Synonyms: hUbc12, NEDD8 carrier protein, NEDD8 conjugating enzyme Ubc12, NEDD8 protein ligase, NEDD8-conjugating enzyme Ubc12, UBC-RS2, UBC12, UBC12_HUMAN, ube2m, ubiquitin carrier protein M, Ubiquitin conjugating enzyme E2 M, Ubiquitin-conjugating enzyme E2 M, ubiquitin-protein ligase M, yeast UBC12 homolog.

Background: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.

Gene ID: 9040