PAPOL A+B+G antibody
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- Target
- PAPOL A+B+G
- Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human PAPOLA/PAPOLB/PAPOLG
- Isotype
- IgG
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-
- Application Notes
-
WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
-
- Target
- PAPOL A+B+G
- Background
-
Synonyms: PAPOL A/B/G, PAP alpha/beta/gamma, PapIII, MGC5378, PAP alpha, PAP beta, PAP gamma, PAP, PAP-alpha, PAP-beta, PAP-gamma, PAPOLA, PAPOLB, PAPOLG, PAPOA_HUMAN, PAPOB_HUMAN, PAPOG_HUMAN, Plap, polyA polymerase alpha, Polynucleotide adenylyltransferase alpha, polyA polymerase beta testis specic, polyA polymerase beta, polynucleotide adenylyltransferase beta, TPAP, Testis-specic polyA polymerase.
Background: Polyadenylation of the 3-prime ends of eukaryotic mRNAs is a key event that takes place in the nucleus during maturation of mRNA. The reaction includes endoribonucleolytic cleavage of the pre-RNA at the poly(A) site that leads to synthesis of the poly(A) tail at the 3-prime end of the upstream cleavage product. The poly(A) polymerase (PAP) is required The adenosine addition reaction depends on poly(A) polymerase (PAP) activity. The testis express PAP-beta (TPAP) in the cytoplasm of spermatogenic cells. The adenosine addition function of PAP-beta plays a critical role in male germ cell production. PAP-beta-deficient transgenic mice display impaired expression of haploid-specific genes that are necessary for spermatogenesis. The intronless gene encoding human PAP-beta maps to chromosome 7p22.3.
- Gene ID
- 10914, 56903, 64895
- UniProt
- P51003, Q9NRJ5, Q9BWT3
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