RLIM antibody (AA 550-600)

Catalog No. ABIN1385938

Product Details anti-RLIM Antibody

Target: RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

Binding Specificity: AA 550-600

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RLIM antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RNF12

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for RLIM

Target: RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

Alternative Name: RNF12 (RLIM Products)

Synonyms: NY-REN-43 antibody, RNF12 antibody, AL022832 antibody, AW743871 antibody, Ha1r antibody, Rnf12 antibody, R-LIM antibody, ring finger protein, LIM domain interacting antibody, XRnf12C protein antibody, RLIM antibody, Rlim antibody, xrnf12c antibody, rlim antibody

Background:

Synonyms: RNF12, NY-REN-43, E3 ubiquitin-protein ligase RLIM, LIM domain-interacting RING finger protein, RING finger LIM domain-binding protein, R-LIM, RING finger protein 12, Renal carcinoma antigen NY-REN-43, RLIM

Background: E3 ubiquitin-protein ligase. Acts as a negative coregulator for LIM homeodomain transcription factors by mediating the ubiquitination and subsequent degradation of LIM cofactors LDB1 and LDB2 and by mediating the recruitment the SIN3a/histone deacetylase corepressor complex. Ubiquitination and degradation of LIM cofactors LDB1 and LDB2 allows DNA-bound LIM homeodomain transcription factors to interact with other protein partners such as RLIM. Plays a role in telomere length-mediated growth suppression by mediating the ubiquitination and degradation of TERF1. By targeting ZFP42 for degradation, acts as an activator of random inactivation of X chromosome in the embryo, a stochastic process in which one X chromosome is inactivated to minimize sex-related dosage differences of X-encoded genes in somatic cells of female placental mammals.

Gene ID: 51132

UniProt: Q9NVW2