DPH2 antibody (AA 401-489)

Catalog No. ABIN1385915

Product Details anti-DPH2 Antibody

Target: DPH2 (Diphthamide Biosynthesis Protein 2 (DPH2))

Binding Specificity: AA 401-489

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPH2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Sheep,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DPH2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DPH2

Target: DPH2 (Diphthamide Biosynthesis Protein 2 (DPH2))

Alternative Name: Dph2 (DPH2 Products)

Synonyms: DPH2L2 antibody, 9130020C19Rik antibody, AI467389 antibody, Dph2l2 antibody, id:ibd5058 antibody, zgc:162269 antibody, DPH2 homolog antibody, DPH2 homolog (S. cerevisiae) antibody, hypothetical protein antibody, DPH2 antibody, Dph2 antibody, dph2 antibody, CAALFM_C400690CA antibody

Background:

Synonyms: Diphthamide biosynthesis like protein 2, Diphthamide biosynthesis protein 2, Diphthamide biosynthesis protein 2 homolog like 2, Diphthamide biosynthesis protein 2 homolog-like 2, Diptheria toxin resistance protein required for diphthamide biosynthesis like 2, Diptheria toxin resistance protein required for diphthamide biosynthesis like 2 S. cerevisiae , DPH 2, DPH2, DPH2 homolog, DPH2 homolog S. cerevisiae, DPH2 like 2, DPH2 like 2 S. cerevisiae, DPH2-like 2, DPH2_HUMAN, DPH2L2, HsDph2, OTTHUMP00000010007, Protein DPH2 homolog.

Background: DPH2 (diphthamide biosynthesis protein 2), also known as DPH2L2, is a 489 amino acid protein that shows strong expression in skeletal muscle, moderate expression in heart, small intestine, liver, pancreas, testis and colon, and lesser expression in brain, placenta, kidney, spleen, thymus, prostate, ovary and lymphocytes. DPH2 interacts with DPH1 and, functioning together as a dimer or multimer, DPH1 and DPH2 may participate in diphthamide biosynthesis. Diphthamide is a posttranslationally modified histidine residue which occurs in EF-2 (elongation factor 2) and targets diphtheria toxin ADP-ribosylation. The loss of DPH2 in Saccharomyces cerevisiae is believed to suppress zymocicity. Two transcript variants encoding different isoforms have been found for this gene.Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2.Tissue specificity:Strongly expressed in skeletal muscle. Moderately expressed in heart, small intestine, liver, pancreas, testis and colon. Weakly expressed in brain, placenta, kidney, speen, thymus, prostate, ovary and lymphocytes.

Gene ID: 1802