PCYT2 antibody (AA 315-389)

Catalog No. ABIN1385871

Product Details anti-PCYT2 Antibody

Target: PCYT2 (Phosphate Cytidylyltransferase 2, Ethanolamine (PCYT2))

Binding Specificity: AA 315-389

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCYT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PCYT2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PCYT2

Target: PCYT2 (Phosphate Cytidylyltransferase 2, Ethanolamine (PCYT2))

Alternative Name: PCYT2 (PCYT2 Products)

Synonyms: ET antibody, 1110033E03Rik antibody, ethanolamine-phosphate cytidylyltransferase antibody, putative ethanolamine-phosphate cytidylyltransferase antibody, Ethanolamine-phosphate cytidylyltransferase antibody, phosphate cytidylyltransferase 2, ethanolamine antibody, phosphate cytidylyltransferase 2, ethanolamine L homeolog antibody, Tc00.1047053511727.120 antibody, Tb11.01.5730 antibody, LINJ_32_0940 antibody, LOC5566800 antibody, LMJF_32_0890 antibody, EDI_013070 antibody, EDI_204270 antibody, CpipJ_CPIJ009320 antibody, Bm1_01855 antibody, pcy2 antibody, PCYT2 antibody, Pcyt2 antibody, pcyt2.L antibody

Background:

Synonyms: CTP, phosphoethanolamine cytidylyltransferase, ET, Ethanolamine-phosphate cytidylyltransferase, Ethanolaminephosphate Cytidyltransferase, PCY2, PCY2_HUMAN, Pcyt2, Phosphate cytidylyltransferase 2 ethanolamine, Phosphorylethanolamine transferase.

Background: Phosphatidylethanolamine (PtdEtn) is a major membrane phospholipid which serves to play a primary role in cell membrane structure and is also involved in cell division, cell signaling, activation, phagocytosis and autophagy. PCYT2 (Phosphorylethanolamine transferase), also known as Ethanolamine-phosphate cytidylyltransferase, is a 389 amino acid protein that catalyzes the formation of CDP-ethanolamine from ethanolamine. This product combined with diacylglycerol form phosphatidylethanolamine via the de novo Kennedy pathway. PCYT2 is expressed at highest levels in heart, liver and skeletal muscle. Elevated levels of MyoD, reduced content of Sp1 and a changed ratio of Sp1 to Sp3 all together stimulate upregulation of PCTY2 transcription during C2C12 muscle cell differentiation. Disruption of the PCYT2 gene in mice leads to death after embryo implantation, establishing the necessity of PCYT2 for murine development.