NOS1AP antibody (AA 128-170)

Catalog No. ABIN1385736

Product Details anti-NOS1AP Antibody

Target: NOS1AP (Nitric Oxide Synthase 1 (Neuronal) Adaptor Protein (NOS1AP))

Binding Specificity: AA 128-170

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS1AP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Cow,Sheep,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CAPON

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for NOS1AP

Target: NOS1AP (Nitric Oxide Synthase 1 (Neuronal) Adaptor Protein (NOS1AP))

Alternative Name: CAPON (NOS1AP Products)

Synonyms: MGC78853 antibody, Capon antibody, MGC145362 antibody, 6330408P19Rik antibody, CAPON antibody, nitric oxide synthase 1 adaptor protein antibody, nitric oxide synthase 1 adaptor protein L homeolog antibody, nitric oxide synthase 1 (neuronal) adaptor protein a antibody, carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein antibody, chromosome 18 C1orf226 homolog antibody, nitric oxide synthase 1 (neuronal) adaptor protein antibody, NOS1AP antibody, nos1ap.L antibody, nos1apa antibody, LOC609484 antibody, nos1ap antibody, LOC100058633 antibody, C18H1orf226 antibody, Nos1ap antibody

Background:

Synonyms: NOS1AP, C terminal PDZ domain ligand of neuronal nitric oxide synthase CAPON, C terminal PDZ domain ligand of neuronal nitric oxide synthase, C terminal PDZ ligand of neuronal nitric oxide synthase protein, C-terminal PDZ ligand of neuronal nitric oxide synthase protein, CAPON, CAPON_HUMAN, Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein, Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein, Ligand of neuronal nitric oxide synthase with carboxyl terminal PDZ domain, MGC138500, Nitric oxide synthase 1 neuronal adaptor protein, Nitric oxide synthase 1 adaptor protein.

Background: CAPON (carboxy-terminal PDZ ligand of nNOS) selectively binds within the 100 amino acid PDZ domain of the neuronal nitric oxide synthase (nNOS), but not to endothelial NOS or inducible NOS, and sequesters nNOS in the cytosol. Biosynthesis of the neurotransmitter nitric oxide (NO) requires the association of nNOS with various synaptic proteins, including syntrophin, postsynaptic density (PSD)95 and PSD93 through a scaffolding PDZ domain. These proteins facilitate the transport of nNOS to the plasma membrane, where it is catalytically activated by NMDA-receptor mediated calcium channels. The association of nNOS with PSD95 or PSD93 is regulated by CAPON. The carboxy terminus of CAPON binds to the PDZ domain, competes with PSD95 and PSD93 for binding to nNOS and in turn prevents the translocation and catalytic activation of nNOS.