HEY2 antibody

Catalog No. ABIN1385664

Product Details anti-HEY2 Antibody

Target: HEY2 (Hairy/enhancer-of-Split Related with YRPW Motif 2 (HEY2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HEY2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HEY2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for HEY2

Target: HEY2 (Hairy/enhancer-of-Split Related with YRPW Motif 2 (HEY2))

Alternative Name: HEY2 (HEY2 Products)

Synonyms: CHF1 antibody, GRIDLOCK antibody, GRL antibody, HERP1 antibody, HESR2 antibody, HRT2 antibody, bHLHb32 antibody, Herp1 antibody, Hrt2 antibody, hesr2 antibody, grl antibody, zgc:136746 antibody, hes related family bHLH transcription factor with YRPW motif 2 antibody, hairy/enhancer-of-split related with YRPW motif 2 antibody, hes-related family bHLH transcription factor with YRPW motif 2 antibody, HEY2 antibody, Hey2 antibody, hey2 antibody

Background:

Synonyms: bHLHb32, Cardiovascular helix loop helix factor 1, Cardiovascular helix-loop-helix factor 1, CHF1, Class B basic helix-loop-helix protein 32, GRIDLOCK, GRL, Hairy and enhancer of split related 2, Hairy and enhancer of split-related protein 2, Hairy related transcription factor 2, Hairy-related transcription factor 2, Hairy/enhancer-of-split related with YRPW mot protein 2, hCHF1, HERP, HERP1, HES related repressor protein 1, HES-related repressor protein 2, HESR-2, HESR2, HEY2, HEY2_HUMAN, hHRT2, HRT-2, HRT2, Protein gridlock homolog.

Background: The LIN-12/Notch family of transmembrane receptors plays a central role in development by regulating cell fate and establishing boundaries of gene expression. Notch signaling activates the Hairy/Enhancer of split (HES) genes, which encode basic helix-loop-helix (bHLH) transcriptional repressors that are critical for directing embryonic patterning and development. The Hairy-related transcription factors (HRTs) comprise a subclass of bHLH proteins that exhibit structural similarity with the HES proteins and include HRT1, HRT2 and HRT3. The HRT family (also designated Hesr, Hey, CHF and Gridlock) contain a bHLH domain, an Orange domain and a novel YRPW domain, which is absent in HRT3. The Hairy-related genes map to human chromosomes 8q21, 6q21 and 1p34.3 for HRT1, HRT2 and HRT3, respectively, and are downstream targets for Notch signaling. HRT1 is expressed in the somitic mesoderm, central nervous system, kidney, heart, nasal epithelium and limb buds in murine embryos as well as in adult tissues. It has altered expression in many breast, lung and kidney tumors. Like HRT1, HRT2 and HRT3 are also expressed in developing somites, heart and nervous system.

Gene ID: 23493

Pathways: Regulation of Muscle Cell Differentiation