DCAKD antibody (AA 61-160)

Catalog No. ABIN1385641

Product Details anti-DCAKD Antibody

Target: DCAKD (dephospho-CoA Kinase Domain Containing (DCAKD))

Binding Specificity: AA 61-160

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCAKD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DCAKD

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DCAKD

Target: DCAKD (dephospho-CoA Kinase Domain Containing (DCAKD))

Alternative Name: Dcakd (DCAKD Products)

Synonyms: 3010024O21Rik antibody, 6720485C15Rik antibody, AI849483 antibody, MGC153150 antibody, zgc:153150 antibody, dephospho-CoA kinase domain containing antibody, DCAKD antibody, Dcakd antibody, dcakd antibody

Background:

Synonyms: Dephospho CoA kinase domain containing protein, Dephospho CoA kinase domain containing, isoform CRA_b, DCAKD_HUMAN.

Background: DCAKD belongs to the coaE family. It contains one DPCK (dephospho CoA kinase) domain. There are two isoforms.Coenzyme A (CoA) is an essential cofactor used in numerous biochemical pathways. It plays a critical role in the synthesis and oxidation of fatty acids and is vital to the citric acid cycle. The biosynthesis pathway of CoA from pantothenic acid (also known as vitamin B5) is essential and universal in prokaryotes and eukaryotes. In humans, the final steps of the biosynthesis pathway are carried out by the bifunctional enzyme COASY. The sequence of these enzymes are highly conserved between different bacterial species. The phosphopantetheine adenylyltransferase and decoenzyme A kinase activities of COASY are evolutionarily conserved activities. DCAKD (deCoA kinase domain containing protein) is a 231 amino acid protein that consists of a deCoA kinase domain and an ATP nucleotide binding motif. Localizing to mitochondria and the cytosol, DCAKD belongs to the coaE family which suggests that it may play a role in the biosynthesis of CoA.

Gene ID: 79877

Pathways: SARS-CoV-2 Protein Interactome