CD73 antibody (AA 151-250)

Catalog No. ABIN1385637

Product Details anti-CD73 Antibody

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Binding Specificity: AA 151-250

Reactivity: Human, Mouse, Rat, Cow, Dog, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD73 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat, Sheep

Predicted Reactivity: Cow,Pig,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CD73/NT5

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD73 antibody (ABIN1385637)

Ma, Liu, Li, Lu, Bai, Fan, Guan, Guo: "Cryopreservation and multipotential characteristics evaluation of a novel type of mesenchymal stem cells derived from Small Tailed Han Sheep fetal lung tissue." in: Cryobiology, Vol. 75, pp. 7-14, (2017) (PubMed).

Long, Luo, Wang, Bates, Shetty: "Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells." in: Aging and disease, Vol. 8, Issue 3, pp. 301-313, (2017) (PubMed).

Lin, Fujita, Endo, Morita, Takeda, Nakagawa, Nishimura: "Isolation and Characterization of Multipotent Mesenchymal Stem Cells Adhering to Adipocytes in Canine Bone Marrow." in: Stem cells and development, Vol. 26, Issue 6, pp. 431-440, (2016) (PubMed).

Long, Li, Luo, Hei, Wang, Tian, Yang, Lei, Qiu, Liu: "MRI tracking of bone marrow mesenchymal stem cells labeled with ultra-small superparamagnetic iron oxide nanoparticles in a rat model of temporal lobe epilepsy." in: Neuroscience letters, Vol. 606, pp. 30-5, (2015) (PubMed).

Wang, Chen, Wu, Zhang, Hu, Ge, Yang, Xu, Liu, Ye: "Small activating RNA induces myogenic differentiation of rat adipose-derived stem cells by upregulating MyoD." in: International braz j urol : official journal of the Brazilian Society of Urology, Vol. 41, Issue 4, pp. 764-72, (2015) (PubMed).

Long, Hei, Luo, Tian, Yang, Li, Wei, Liu: "BMSCs transplantation improves cognitive impairment via up-regulation of hippocampal GABAergic system in a rat model of chronic cerebral hypoperfusion." in: Neuroscience, Vol. 311, pp. 464-73, (2015) (PubMed).

Gao, Bai, Wang, Sun, Guan, Zheng: "All-trans retinoic acid promotes nerve cell differentiation of yolk sac-derived mesenchymal stem cells." in: Applied biochemistry and biotechnology, Vol. 174, Issue 2, pp. 682-92, (2014) (PubMed).

Lu, Xiong, Wang, Wang, Ma, Guan: "Isolation and characterization of adipose-derived mesenchymal stem cells (ADSCs) from cattle." in: Applied biochemistry and biotechnology, Vol. 174, Issue 2, pp. 719-28, (2014) (PubMed).

Gao, Zhang, Ding, Chen, Hu, Jiang, Li, Chen, Wang, Ye, Zhu: "Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells." in: Cell transplantation, Vol. 22, Issue 10, pp. 1883-900, (2014) (PubMed).

Gao, Bai, Xiong, Li, Shan, Huang, Ma, Guan: "Isolation and characterization of chicken dermis-derived mesenchymal stem/progenitor cells." in: BioMed research international, Vol. 2013, pp. 626258, (2013) (PubMed).

Long, Qiu, Wang, Yang, Jia, Xin, Wang, Han, Fei, Liu: "Genetically engineered bone marrow mesenchymal stem cells improve functional outcome in a rat model of epilepsy." in: Brain research, Vol. 1532, pp. 1-13, (2013) (PubMed).

Wang, Long, Jia, Liu, Yi, Yang, Fei, Liu: "Over-expression of Mash1 improves the GABAergic differentiation of bone marrow mesenchymal stem cells in vitro." in: Brain research bulletin, Vol. 99, pp. 84-94, (2013) (PubMed).

Takemitsu, Zhao, Yamamoto, Harada, Michishita, Arai: "Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells." in: BMC veterinary research, Vol. 8, Issue 1, pp. 150, (2012) (PubMed).

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: CD73 (NT5E Products)

Synonyms: CALJA antibody, CD73 antibody, E5NT antibody, NT antibody, NT5 antibody, NTE antibody, eN antibody, eNT antibody, zgc:63784 antibody, NT5E antibody, MGC114869 antibody, PSPTO2277 antibody, 2210401F01Rik antibody, AI447961 antibody, Nt5 antibody, 5'-nucleotidase ecto antibody, 5'-nucleotidase, ecto (CD73) antibody, 5'-nucleotidase antibody, 5' nucleotidase, ecto antibody, NT5E antibody, nt5e antibody, nt5e.S antibody, PSPTO_2277 antibody, CBU_1233 antibody, MCA1322 antibody, Nt5e antibody

Background:

Synonyms: NT, eN, NT5, NTE, eNT, CD73, E5NT, CALJA, 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, NT5E

Background: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Gene ID: 4907

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process