Nitrotyrosine antibody

Catalog No. ABIN1385080

Product Details anti-Nitrotyrosine Antibody

Target: Nitrotyrosine

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nitrotyrosine antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Nitrotyrosine

Purification: Purified by Protein A.

Immunogen: KLH conjugated to Nitrotyrosine

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Nitrotyrosine antibody (ABIN1385080)

Li, Du, Yuan, Han, Dong, Chen, Wu, Zhang, Xu, Han, Zhang, Xia: "Hepatic hypoxia-inducible factors inhibit PPARα expression to exacerbate acetaminophen induced oxidative stress and hepatotoxicity." in: Free radical biology & medicine, Vol. 110, pp. 102-116, (2017) (PubMed).

Zhang, Song, Pang, Zhang, Wan, Yuan, Wu, Liu: "Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice." in: World journal of gastroenterology : WJG, Vol. 21, Issue 14, pp. 4195-209, (2015) (PubMed).

Chen, Kan: "Down-regulation of superoxide dismutase 1 by PMA is involved in cell fate determination and mediated via protein kinase D2 in myeloid leukemia cells." in: Biochimica et biophysica acta, Vol. 1853, Issue 10 Pt A, pp. 2662-75, (2015) (PubMed).

Wang, Su, Wang, Zhang, Zhang, Niu, Guo, Li, Jiang, Liu, Guo: "Tert-butylhydroquinone ameliorates doxorubicin-induced cardiotoxicity by activating Nrf2 and inducing the expression of its target genes." in: American journal of translational research, Vol. 7, Issue 10, pp. 1724-35, (2015) (PubMed).

Vendrov, Vendrov, Smith, Yuan, Sumida, Robidoux, Runge, Madamanchi: "NOX4 NADPH Oxidase-Dependent Mitochondrial Oxidative Stress in Aging-Associated Cardiovascular Disease." in: Antioxidants & redox signaling, (2015) (PubMed).

Target Details for Nitrotyrosine

Target: Nitrotyrosine

Abstract: Nitrotyrosine Products

Target Type: Chemical

Background:

Synonyms: NULL

Background: Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques and chronic renal failure.