CD25 antibody

Catalog No. ABIN1383666

Product Details anti-CD25 Antibody

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD25 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Functional Studies (Func)

Specificity: Recognises the Interleukin 2 Receptor a chain (IL-2 Ra), a 55 kDa protein

Purification: Ion exchange chromatography

Sterility: 0.22 μm filtered

Immunogen: PHA activated T cells,Myeloma X63/AG.8653 x Balb/c spleen cells

Clone: B-B10

Isotype: IgG1 kappa

Biological Activity Comment: Inhibits mixed lymphocyte culture

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate-buffered saline. Sterile-filtered through 0.22 μm. Carrier and preservative free

Preservative: Without preservative

Storage: 4 °C

Storage Comment: Stable at +2-8°C for 12 months. For longer storage freeze aliquots.

References for anti-CD25 antibody (ABIN1383666)

Regeer, Markovich: "A dileucine motif targets the sulfate anion transporter sat-1 to the basolateral membrane in renal cell lines." in: American journal of physiology. Cell physiology, Vol. 287, Issue 2, pp. C365-72, (2004) (PubMed).

Groux, Bigler, de Vries, Roncarolo: "Inhibitory and stimulatory effects of IL-10 on human CD8+ T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 7, pp. 3188-93, (1998) (PubMed).

Nevill, Fung, Shepherd, Horsman, Nantel, Klingemann, Forrest, Toze, Sutherland, Hogge, Naiman, Le, Brockington, Barnett: "Cytogenetic abnormalities in primary myelodysplastic syndrome are highly predictive of outcome after allogeneic bone marrow transplantation." in: Blood, Vol. 92, Issue 6, pp. 1910-7, (1998) (PubMed).

Ozsahin, von Planta, Müller, Steinert, Nadal, Lauener, Tuchschmid, Willi, Ozsahin, Crompton, Seger: "Successful treatment of invasive aspergillosis in chronic granulomatous disease by bone marrow transplantation, granulocyte colony-stimulating factor-mobilized granulocytes, and liposomal amphotericin-B." in: Blood, Vol. 92, Issue 8, pp. 2719-24, (1998) (PubMed).

Agostini, Trentin, Sancetta, Facco, Tassinari, Cerutti, Bortolin, Milani, Siviero, Zambello, Semenzato: "Interleukin-15 triggers activation and growth of the CD8 T-cell pool in extravascular tissues of patients with acquired immunodeficiency syndrome." in: Blood, Vol. 90, Issue 3, pp. 1115-23, (1997) (PubMed).

Racadot, Mousson, Tanter, Wijdenes, Rifle, Herve: "Outcome of lymphocyte subsets and cytokines during combined CD4/anti-IL-2R monoclonal antibody therapy in kidney transplantation." in: Transplantation proceedings, Vol. 27, Issue 2, pp. 1672-3, (1995) (PubMed).

Racadot, Milpied, Bordigoni, Cahn, Plouvier, Lioure, Lutz, Wijdenes, Herve: "Sequential use of three monoclonal antibodies in corticosteroid-resistant acute GVHD: a multicentric pilot study including 15 patients." in: Bone marrow transplantation, Vol. 15, Issue 5, pp. 669-77, (1995) (PubMed).

Mousson, Tanter, Racadot, Wijdenes, Chalopin, Chevet, Hervé, Rifle: "CD4 and CD25 monoclonal antibody cocktail in kidney transplant rejection prophylaxis: clinical results of a pilot study." in: Transplantation proceedings, Vol. 27, Issue 2, pp. 1727-8, (1995) (PubMed).

Herbelin, Stephan, Donadieu, Le Deist, Racadot, Wijdenes, Fischer: "Treatment of steroid-resistant acute graft-versus-host disease with an anti-IL-2-receptor monoclonal antibody (BT 563) in children who received T cell-depleted, partially matched, related bone marrow transplants." in: Bone marrow transplantation, Vol. 13, Issue 5, pp. 563-9, (1994) (PubMed).

Nakatani, Lone, Yamakawa, Kanaoka, Gomi, Wijdenes, Noguchi: "Humanization of mouse anti-human IL-2 receptor antibody B-B10." in: Protein engineering, Vol. 7, Issue 3, pp. 435-43, (1994) (PubMed).

Tazzari, Bolognesi, De Totero, Pileri, Conte, Wijdenes, Hervé, Soria, Stirpe, Gobbi: "B-B10 (anti-CD25)-saporin immunotoxin--a possible tool in graft-versus-host disease treatment." in: Transplantation, Vol. 54, Issue 2, pp. 351-6, (1992) (PubMed).

Heyll, Aul, Söhngen, Meckenstock, Arning, Wurm, Schmitt, Schneider: "[Therapy of multiple myeloma by allogeneic bone marrow transplantation]." in: Deutsche medizinische Wochenschrift (1946), Vol. 116, Issue 22, pp. 852-6, (1991) (PubMed).

Engert, Martin, Amlot, Wijdenes, Diehl, Thorpe: "Immunotoxins constructed with anti-CD25 monoclonal antibodies and deglycosylated ricin A-chain have potent anti-tumour effects against human Hodgkin cells in vitro and solid Hodgkin tumours in mice." in: International journal of cancer. Journal international du cancer, Vol. 49, Issue 3, pp. 450-6, (1991) (PubMed).

Tiberghien, Racadot, Lioure, Delain, Girard, Wijdenes, Plouvier, Flesch, Cahn, Herve: "Soluble CD8, IL-2 receptor, and tumor necrosis factor-alpha levels in steroid-resistant acute graft-versus-host disease. Relation with subsequent response to anti-IL-2 receptor monoclonal antibody treatment." in: Transplantation, Vol. 52, Issue 3, pp. 475-80, (1991) (PubMed).

Wijdenes, Beliard, Muot, Herve, Peters: "A semi-pharmaceutical approach for the preparation of an anti-IL2 receptor monoclonal antibody in the treatment of acute GvHD in a multicentric study." in: Developments in biological standardization, Vol. 71, pp. 103-11, (1990) (PubMed).

Hervé, Wijdenes, Bergerat, Bordigoni, Milpied, Cahn, Clément, Béliard, Morel-Fourrier, Racadot: "Treatment of corticosteroid resistant acute graft-versus-host disease by in vivo administration of anti-interleukin-2 receptor monoclonal antibody (B-B10)" in: Blood, Vol. 75, Issue 4, pp. 1017-23, (1990) (PubMed).

Hervé, Wijdenes, Bergerat, Milpied, Gaud, Bordigoni: "Treatment of acute graft-versus-host disease with monoclonal antibody to IL-2 receptor." in: Lancet, Vol. 2, Issue 8619, pp. 1072-3, (1988) (PubMed).

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25 (IL2RA Products)

Synonyms: CD25 antibody, IDDM10 antibody, IL2R antibody, TCGFR antibody, IL2RAC antibody, IL-2RA antibody, Il2r antibody, Ly-43 antibody, interleukin 2 receptor subunit alpha antibody, interleukin-2 receptor subunit alpha-like antibody, interleukin 2 receptor, alpha chain antibody, IL2RA antibody, Il2ra antibody, LOC100222957 antibody

Background: IL-2R α-Chain

Molecular Weight: 55 kDa

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation