CD35 antibody

Catalog No. ABIN135765

Product Details anti-CD35 Antibody

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD35 antibody is un-conjugated

Application: Flow Cytometry (FACS), DNA Microscopy (DNA Mic)

Specificity: Human/Rhesus/Cynomolgus/Chimpanzee/Baboon CD35, Mr 160, 190, 220, 250 kDa

Characteristics: Mouse Anti-Human CD35-UNLB

Purification: Purified

Immunogen: Acute monocytic leukemia cells and normal blood monocytes

Clone: E11

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , WB - Reported in literature , ELISA - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC and PE conjugate 10 L/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD35 antibody (ABIN135765)

Avanzi, Leoni, Rotola, Alviano, Solimando, Lanzoni, Bonsi, Di Luca, Marchionni, Alvisi, Ripalti: "Susceptibility of human placenta derived mesenchymal stromal/stem cells to human herpesviruses infection." in: PLoS ONE, Vol. 8, Issue 8, pp. e71412, (2013) (PubMed).

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: CD35 (CR1 Products)

Synonyms: C3BR antibody, C4BR antibody, CD35 antibody, KN antibody, C3DR antibody, CD21 antibody, Cr-1 antibody, Cr-2 antibody, Cr1 antibody, CR1-L antibody, CR2 antibody, CR1 antibody, complement C3b/C4b receptor 1 (Knops blood group) antibody, complement receptor 2 antibody, complement component (3d/Epstein Barr virus) receptor 2 antibody, complement receptor type 1 antibody, CR1 antibody, Cr2 antibody, CR2 antibody, LOC718120 antibody

Background: The CD35 antigen is composed of four different allotypes having different molecular weights (A (or F) - 160 kDa, B (or S) - 190 kDa, C - 220 kDa, and D - 250 kDa). It is the receptor for C3b and is found on B cells, a subset of T cells, monocytes, neutrophils, follicular dendritic cells, erythrocytes and kidney glomelular podocytes. CD35 mediates neutrophil and monocyte phagocytosis of particles coated with C3b and/or C4b. This interaction also enhances phagocytosis mediated by the Fc receptors.

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity