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Bu-1 antibody (Biotin)

Reactivity: Chicken FACS Host: Mouse Monoclonal AV20 Biotin
Catalog No. ABIN135596
  • Target See all Bu-1 products
    Bu-1
    Reactivity
    Chicken
    Host
    • 9
    Mouse
    Clonality
    • 9
    Monoclonal
    Conjugate
    • 3
    • 3
    • 2
    • 1
    This Bu-1 antibody is conjugated to Biotin
    Application
    • 9
    • 6
    • 6
    • 5
    Flow Cytometry (FACS)
    Specificity
    Chicken Bu-1, Mr 70 kDa
    Characteristics
    Mouse Anti-Chicken Bu-1-BIOT
    Immunogen
    Chicken peripheral blood lymphocytes and splenocytes
    Clone
    AV20
    Isotype
    IgG1
  • Application Notes
    • Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature
    • Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells AF647 conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
    Sample Volume
    1 mL
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    0.5 mg in 1.0 mL of PBS/Sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect conjugated products from light.
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C
  • Mucksová, Plachý, Staněk, Hejnar, Kalina, Benešová, Trefil: "Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines." in: Veterinary research, Vol. 48, Issue 1, pp. 18, (2017) (PubMed).

  • Target
    Bu-1
    Abstract
    Bu-1 Products
    Synonyms
    B6.2 antibody, Bu-1 antibody, Bu-1b antibody, B6.1 antibody, LOC396098 antibody
    Background
    Chicken Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated, disulfide-linked homodimer. MAb AV20 recognizes a monomorphic determinant on the Bu-1 B cell-associated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85-90 % of bursal cells, 2-8 % of thymocytes, 15-27 % of spleen cells, and 2-18 % of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.
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