Bu-1 antibody (Biotin)
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- Target See all Bu-1 products
- Bu-1
- Reactivity
- Chicken
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Bu-1 antibody is conjugated to Biotin
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Application
- Flow Cytometry (FACS)
- Specificity
- Chicken Bu-1, Mr 70 kDa
- Characteristics
- Mouse Anti-Chicken Bu-1-BIOT
- Immunogen
- Chicken peripheral blood lymphocytes and splenocytes
- Clone
- AV20
- Isotype
- IgG1
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- Application Notes
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- Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature
- Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells AF647 conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
- Sample Volume
- 1 mL
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- 0.5 mg in 1.0 mL of PBS/Sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C
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Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines." in: Veterinary research, Vol. 48, Issue 1, pp. 18, (2017) (PubMed).
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Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines." in: Veterinary research, Vol. 48, Issue 1, pp. 18, (2017) (PubMed).
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- Target
- Bu-1
- Abstract
- Bu-1 Products
- Synonyms
- B6.2 antibody, Bu-1 antibody, Bu-1b antibody, B6.1 antibody, LOC396098 antibody
- Background
- Chicken Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated, disulfide-linked homodimer. MAb AV20 recognizes a monomorphic determinant on the Bu-1 B cell-associated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85-90 % of bursal cells, 2-8 % of thymocytes, 15-27 % of spleen cells, and 2-18 % of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.
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