Bu-1 antibody

Catalog No. ABIN135594

Product Details anti-Bu-1 Antibody

Target: Bu-1

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Bu-1 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: Chicken Bu-1, Mr 70 kDa

Characteristics: Mouse Anti-Chicken Bu-1-UNLB

Purification: Purified

Immunogen: Chicken peripheral blood lymphocytes and splenocytes

Clone: AV20

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells AF647 conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-Bu-1 antibody (ABIN135594)

Ching, Collarini, Abdiche, Bedinger, Pedersen, Izquierdo, Harriman, Zhu, Etches, van de Lavoir, Harriman, Leighton: "Chickens with humanized immunoglobulin genes generate antibodies with high affinity and broad epitope coverage to conserved targets." in: mAbs, Vol. 10, Issue 1, pp. 71-80, (2019) (PubMed).

Zhang, Sun, Wang, Chen, Huang, Chen, Chen: "The Postembryonic Development of the Immunological Barrier in the Chicken Spleens." in: Journal of immunology research, Vol. 2019, pp. 6279360, (2019) (PubMed).

Target Details for Bu-1

Target: Bu-1

Abstract: Bu-1 Products

Synonyms: B6.2 antibody, Bu-1 antibody, Bu-1b antibody, B6.1 antibody, LOC396098 antibody

Background: Chicken Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated, disulfide-linked homodimer. MAb AV20 recognizes a monomorphic determinant on the Bu-1 B cell-associated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85-90 % of bursal cells, 2-8 % of thymocytes, 15-27 % of spleen cells, and 2-18 % of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.