Bu-1b antibody
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- Target See all Bu-1b products
- Bu-1b (Bu-1b (B Cell Surface Alloantigens) (Bu-1b))
- Reactivity
- Chicken
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Bu-1b antibody is un-conjugated
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Application
- Flow Cytometry (FACS)
- Specificity
- Chicken Bu-1b, Mr 70 kDa
- Characteristics
- Mouse Anti-Chicken Bu-1b-UNLB
- Purification
- Purified
- Immunogen
- Bursa cells from two- to five-week-old CHA (Bu-1b) strain chickens
- Clone
- 5-11G2
- Isotype
- IgG1
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anti-Bu-1b (B Cell Surface Alloantigens) (Bu-1b) antibody (PE)
Bu-1b Reactivity: Chicken FACS Host: Mouse Monoclonal 5-11G2 PE
anti-Bu-1b (B Cell Surface Alloantigens) (Bu-1b) antibody (Biotin)Bu-1b Reactivity: Chicken FACS Host: Mouse Monoclonal 5-11G2 Biotin
anti-Bu-1b (B Cell Surface Alloantigens) (Bu-1b) antibody (FITC)Bu-1b Reactivity: Chicken FACS Host: Mouse Monoclonal 5-11G2 FITC
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- Application Notes
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- Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Apop - Reported in literature
- Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC and BIOT conjugates 1 g/106 cells PE conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
- Sample Volume
- 1 mL
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
- Preservative
- Without preservative
- Handling Advice
- Each reagent is stable for the period shown on the bottle label if stored as directed.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C
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- Target
- Bu-1b (Bu-1b (B Cell Surface Alloantigens) (Bu-1b))
- Alternative Name
- Bu-1b (Bu-1b Products)
- Synonyms
- B6.2 antibody, Bu-1 antibody, Bu-1b antibody, B6.1 antibody, LOC396098 antibody
- Background
- Chicken Bu-1b, a product of the Bu-1b allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90 % of bursal cells, 2-8 % of thymocytes, 15-27 % of spleen cells, and 2-18 % of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion.
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