LY6C + LY6G antibody (Biotin)

Catalog No. ABIN135405

Product Details

Target: LY6C + LY6G

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Specificity: Mouse Ly-6G/Ly-6C, Mr 21-25 kDa

Characteristics: Rat Anti-Mouse Ly-6G/Ly-6C-BIOT

Immunogen: Unknown

Clone: RB6-8C5

Isotype: IgG2b

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , CMCD - Reported in literature , Depletion - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, AF488, and PACBLU conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells SPRD, APC, CY5, PE/CY5.5, PE/CY7, APC/CY7, and AF700 0.03 g/106 cells conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro complement-dependent cytotoxicity assays, In vivo depletion of Ly-6G+ cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details

Target: LY6C + LY6G

Alternative Name: Ly-6G/Ly-6C

Background: Ly-6G, formerly known as the myeloid differentiation antigen Gr-1, is a GPI-anchored protein. In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. It is also transiently expressed on monocytes in the bone marrow. In the periphery, Ly-6G is expressed predominantly on neutrophils.