CD45RC
Reactivity: Rat
FACS
Host: Mouse
Monoclonal
MRC OX-22
Biotin
Application Notes
Applications: FC - Quality tested
Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Sample Volume
1 mL
Restrictions
For Research Use only
Concentration
0.1 mg/mL
Buffer
0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Do not freeze! Protect conjugated products from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
CD45 antibody, L-CA antibody, Lca antibody, RT7 antibody, T200 antibody, protein tyrosine phosphatase, receptor type, C antibody, Ptprc antibody
Background
The CD45 family of cell-surface glycoprotein antigens exist as a set of isoforms. The patterns of monoclonal antibody recognition of the CD45 phenotypes can be restricted (CD45RA, RB and RC), or broader, such as the CD45 T-200 species. The restricted subsets of this group arise as a result of translation of alternatively spliced mRNAs from exons A, B and C (exons 4, 5 and 6), where exons 3-15 encode the extracellular domain of the CD45 molecule. The restricted epitopes are differentially expressed on T and B lymphocytes, including functionally distinct T cells.