NK-1.1/CD161c antibody

Catalog No. ABIN135344

Product Details anti-NK-1.1/CD161c Antibody

Target: NK-1.1/CD161c (NK-1.1)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NK-1.1/CD161c antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: Mouse NK1.1, Mr 39 kDa

Characteristics: Mouse Anti-Mouse NK1.1-LE/AF

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Endotoxin Level: Low endotoxin

Immunogen: CE mouse spleen enriched for NK-1+ cells and bone marrow cells

Clone: PK136

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC - Reported in literature , IP - Reported in literature , Depletion - Reported in literature , CMCD - Reported in literature , Block - Reported in literature , Activ - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, PACBLU, AF488, and AF700 conjugates 1 g/106 cells PE and APC conjugates 0.5 g/106 cells PE/CY7 and AF647 conjugates 0.3 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Induction of proliferation of a subset of NK cells, In vivo depletion of NK1.1 cells, In vitro redirected lysis, In vitro cytotoxicity assays

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of PBS

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: -20 °C

Storage Comment: Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.

Target Details for NK-1.1/CD161c

Target: NK-1.1/CD161c (NK-1.1)

Alternative Name: NK1.1 (NK-1.1/CD161c Products)

Synonyms: AI462337 antibody, CD161 antibody, Ly-59 antibody, Ly55c antibody, Ly59 antibody, NK-RP1 antibody, NK1.1 antibody, NKR-P1.9 antibody, NKRP1 antibody, NKRP140 antibody, Nk-1 antibody, Nk-1.2 antibody, Nk1 antibody, Nk1.2 antibody, Nkrp1c antibody, ly-55c antibody, killer cell lectin-like receptor subfamily B member 1C antibody, Klrb1c antibody

Background: NK1.1, a member of the NKR-P1 family of cell surface receptors, is a type II integral membrane glycoprotein with a C-type lectin domain. It is expressed as a disulfide-linked homodimer on all NK cells, as well as subsets of thymocytes and peripheral T lymphocytes in selected strains of mice (e.g., C57BL, NZB). NK1.1 mediates cellular activation and differentiation, and is thought to have a particular role in generating Th2 cells. This product does not react with NK cells of BALB/c mice.