NK-1.1/CD161c antibody (FITC)
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- Target See all NK-1.1/CD161c products
- NK-1.1/CD161c (NK-1.1)
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Reactivity
- Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This NK-1.1/CD161c antibody is conjugated to FITC
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Application
- Flow Cytometry (FACS)
- Specificity
- Mouse NK1.1, Mr 39 kDa
- Characteristics
- Mouse Anti-Mouse NK1.1-FITC
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Immunogen
- CE mouse spleen enriched for NK-1+ cells and bone marrow cells
- Clone
- PK136
- Isotype
- IgG2a
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- Application Notes
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- Applications: FC - Quality tested , IHC - Reported in literature , IP - Reported in literature , Depletion - Reported in literature , CMCD - Reported in literature , Block - Reported in literature , Activ - Reported in literature
- Working Dilutions: Flow Cytometry FITC, BIOT, PACBLU, AF488, and AF700 conjugates 1 g/106 cells PE and APC conjugates 0.5 g/106 cells PE/CY7 and AF647 conjugates 0.3 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
- Comment
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Induction of proliferation of a subset of NK cells, In vivo depletion of NK1.1 cells, In vitro redirected lysis, In vitro cytotoxicity assays
- Sample Volume
- 1 mL
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- 0.5 mg in 1.0 mL of PBS/Sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C
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- Target
- NK-1.1/CD161c (NK-1.1)
- Alternative Name
- NK1.1 (NK-1.1/CD161c Products)
- Synonyms
- AI462337 antibody, CD161 antibody, Ly-59 antibody, Ly55c antibody, Ly59 antibody, NK-RP1 antibody, NK1.1 antibody, NKR-P1.9 antibody, NKRP1 antibody, NKRP140 antibody, Nk-1 antibody, Nk-1.2 antibody, Nk1 antibody, Nk1.2 antibody, Nkrp1c antibody, ly-55c antibody, killer cell lectin-like receptor subfamily B member 1C antibody, Klrb1c antibody
- Background
- NK1.1, a member of the NKR-P1 family of cell surface receptors, is a type II integral membrane glycoprotein with a C-type lectin domain. It is expressed as a disulfide-linked homodimer on all NK cells, as well as subsets of thymocytes and peripheral T lymphocytes in selected strains of mice (e.g., C57BL, NZB). NK1.1 mediates cellular activation and differentiation, and is thought to have a particular role in generating Th2 cells. This product does not react with NK cells of BALB/c mice.
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