Applications: FC - Quality tested , ICC - Reported in literature , IP - Reported in literature , WB - Reported in literature , Block - Reported in literature , Sep - Reported in literature , Drug Delivery - Reported in literature
Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Sample Volume
1 mL
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
0.5 mg of purified immunoglobulin in 1.0 mL of PBS
Preservative
Azide free
Handling Advice
Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage
-20 °C
Storage Comment
Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.
CD71, a type II transmembrane glycoprotein expressed on the cell surface as a homodimer, is the transferrin receptor. It is essential for the growth of normal and neoplastic cells. It is expressed at low levels on resting B and T lymphocytes but is upregulated during responses to antigens and mitogens, presumably reflecting the iron dependence of proliferation. Its expression declines with maturation and differentiation. CD71 plays a critical role in cell proliferation by controlling the supply of iron, which is essential for many metabolic pathways, through the binding and endocytosis of transferrin, the major iron-carrying protein. It may also be involved in signal transduction via its association with the T cell receptor chain.