CD69 antibody (FITC)

Catalog No. ABIN135255

Product Details anti-CD69 Antibody (FITC)

Target: CD69

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD69 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: Mouse CD69, Mr 85 kDa (unreduced)

Characteristics: Hamster Anti-Mouse CD69-FITC

Purification: Protein A

Immunogen: Mouse dendritic epidermal cell line Y245

Clone: H1-2F3

Isotype: IgG

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Costim - Reported in literature , Activ - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, PE/CY7, AF647, and PACBLU conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro T cell and NK-cell activation

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD69 antibody (FITC) (ABIN135255)

Waaler, Mygland, Tveita, Strand, Solberg, Olsen, Aizenshtadt, Fauskanger, Lund, Brinch, Lycke, Dybing, Nygaard, Bøe, Heintz, Hovig, Hammarström, Corthay, Krauss: "Tankyrase inhibition sensitizes melanoma to PD-1 immune checkpoint blockade in syngeneic mouse models." in: Communications biology, Vol. 3, Issue 1, pp. 196, (2020) (PubMed).

Target Details for CD69

Target: CD69

Alternative Name: CD69 (CD69 Products)

Synonyms: 5830438K24Rik antibody, AI452015 antibody, AIM antibody, VEA antibody, BL-AC/P26 antibody, CLEC2C antibody, EA1 antibody, GP32/28 antibody, MLR-3 antibody, Cd69 molecule antibody, CD69 antigen antibody, CD69 molecule antibody, Cd69 antibody, CD69 antibody

Background: CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa. It is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils. Although not detectable on resting lymphocytes, its expression is rapidly (within 2 hours) upregulated upon activation of T, B and NK cells, and neutrophils.4 Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells.