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CD3 epsilon antibody

CD3E Reactivity: Mouse FACS Host: Hamster Monoclonal 145-2C11 unconjugated
Catalog No. ABIN135085
  • Target See all CD3 epsilon (CD3E) Antibodies
    CD3 epsilon (CD3E)
    Reactivity
    • 230
    • 157
    • 51
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    Mouse
    Host
    • 139
    • 123
    • 33
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    • 8
    • 1
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    Hamster
    Clonality
    • 230
    • 112
    • 7
    Monoclonal
    Conjugate
    • 138
    • 32
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    This CD3 epsilon antibody is un-conjugated
    Application
    • 252
    • 145
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    Flow Cytometry (FACS)
    Specificity
    Mouse CD3, Mr 25 kDa
    Characteristics
    Hamster Anti-Mouse CD3e-UNLB
    Purification
    Purified
    Immunogen
    H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone BM10-37
    Clone
    145-2C11
    Isotype
    IgG
    Top Product
    Discover our top product CD3E Primary Antibody
  • Application Notes
    • Applications: FC - Quality tested , IHC - Reported in literature , IP - Reported in literature , WB - Reported in literature , Block - Reported in literature , Depletion - Reported in literature , Activ - Reported in literature , CMCD - Reported in literature
    • Working Dilutions: Flow Cytometry FITC conjugate 3 g/106 cells BIMA and AF488 conjugates 1 g/106 cells PE, APC, SPRD, and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 l
    Comment

    In vitro depletion of CD3+ cells, In vitro activation of T cells

    Sample Volume
    1 mL
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added
    Preservative
    Without preservative
    Handling Advice
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C
  • Ershaid, Sharon, Doron, Raz, Shani, Cohen, Monteran, Leider-Trejo, Ben-Shmuel, Yassin, Gerlic, Ben-Baruch, Pasmanik-Chor, Apte, Erez: "NLRP3 inflammasome in fibroblasts links tissue damage with inflammation in breast cancer progression and metastasis." in: Nature communications, Vol. 10, Issue 1, pp. 4375, (2020) (PubMed).

  • Target
    CD3 epsilon (CD3E)
    Alternative Name
    CD3e (CD3E Products)
    Background
    CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.
    Pathways
    TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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