Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , WB - Reported in literature , Adhesion - Reported in literature , Block - Reported in literature
Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Sample Volume
1 mL
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
Preservative
Without preservative
Handling Advice
Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage
4 °C
Storage Comment
Store at 2-8°C
Wang, Cao, Gorshkov, Zhou, Yang, Xu, Ma, Zhang, Wang, Mao, Zeng, Su, Verin, Hong, Liu, Huo: "Ablation of endothelial Pfkfb3 protects mice from acute lung injury in LPS-induced endotoxemia." in: Pharmacological research, Vol. 146, pp. 104292, (2020) (PubMed).
VCAM-1 is an adhesion molecule and a major mediator of the inflammatory response. It is expressed on activated microvascular endothelial cells in response to signals arising from immune responses in infection, graft rejection, tumor recognition and killing. The complementary binding ligand for VCAM-1 is VLA-4/CD49d. In addition to VCAM-1, VLA-4 also recognizes the extracellular matrix molecule fibronectin. This pairing of VCAM-1 and VLA-4 is able to provide a second signal (i.e., non-antigen specific) for T cell stimulation, such as that seen in transplantation. The monoclonal antibody MK-2 has been used in transplant studies to suppress cardiac rejection and induce long-term cardiac graft survival. In addition to inflammatory responses, VCAM-1 has a significant role in hemopoiesis through its ability to retain lymphocyte and myeloid precursors on stromal cells in the marrow and lymphoid organs. CD106/VCAM-1 exists as an integral membrane protein.