Collagen Type V antibody

Catalog No. ABIN135054

Product Details anti-Collagen Type V Antibody

Target: Collagen Type V (COL5) (Collagen, Type V (COL5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Collagen Type V antibody is un-conjugated

Application: ELISA

Specificity: Reacts with conformational determinants on type V collagen Referenced species reactivities include - Human 2,5,6,11,13,14,17,23-26 Chicken 4 Porcine 1,5,8 Mouse 3,12,20 Cuttlefish 27 Rabbit 9,15 Rat 5,7,10,16 Equine 19,21,22 Tree Shrew 18 Canine 5 Guinea Pig 15

Cross-Reactivity (Details): Exhibits < 10 % cross reactivity with collagen type II, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

Characteristics: Goat Anti-Type V Collagen-UNLB

Purification: Purification Method: Affinity chromatography on type V collagen covalently linked to agarose.

Isotype: IgG

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA ,
• Other referenced applications include - IHC-PS , IHC-FS , ICC , EM , WB
• Working Dilutions: ELISA BIOT conjugate 1:1,000 - 1:4,000

Sample Volume: 0,5 mL

Restrictions: For Research Use only

Handling

Concentration: 0.4 mg/mL

Buffer: 0.2 mg of purified immunoglobulin in 0.5 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-Collagen Type V antibody (ABIN135054)

Welc, Flores, Wehling-Henricks, Ramos, Wang, Bertoni, Tidball: "Targeting a therapeutic LIF transgene to muscle via the immune system ameliorates muscular dystrophy." in: Nature communications, Vol. 10, Issue 1, pp. 2788, (2019) (PubMed).

Finno, Gianino, Perumbakkam, Williams, Bordbari, Gardner, Burns, Peng, Durward-Akhurst, Valberg: "A missense mutation in MYH1 is associated with susceptibility to immune-mediated myositis in Quarter Horses." in: Skeletal muscle, Vol. 8, Issue 1, pp. 7, (2018) (PubMed).

Huang, Ge, Izzi, Greenspan: "α3 Chains of type V collagen regulate breast tumour growth via glypican-1." in: Nature communications, Vol. 8, pp. 14351, (2018) (PubMed).

Target Details for Collagen Type V

Target: Collagen Type V (COL5) (Collagen, Type V (COL5))

Alternative Name: Type V Collagen (COL5 Products)