Collagen IV antibody

Catalog No. ABIN135052

Product Details anti-Collagen IV Antibody

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: ELISA

Specificity: Reacts with conformational determinants on type IV collagen Referenced species reactivities include - Human 1,12,14,27,29,31,34,36 Chicken 5,13,15 Porcine 11,22,25 Mouse 4,5,7,8,30,32 Chinchilla 17 Rabbit 5,6,14,20,23 Rat 2,3,10,26,27,33,35 Equine 18,28 Sheep 24 Bovine 5,24 Guinea Pig 14 Tree Shrew 21 Canine 9,19 Japanese Macaque 16

Cross-Reactivity (Details): Exhibits < 10 % cross reactivity with collagen type II, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

Characteristics: Goat Anti-Type IV Collagen-UNLB

Purification: Purification Method: Affinity chromatography on type IV collagen covalently linked to agarose.

Isotype: IgG

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA
• Other referenced applications include - IHC-PS , IHC-FS , ICC , EM , FC , WB , IP
• Working Dilutions: ELISA BIOT conjugate 1:1,000 - 1:4,000 FLISA AF488 conjugate 1:200 - 1:400

Sample Volume: 0,5 mL

Restrictions: For Research Use only

Handling

Concentration: 0.4 mg/mL

Buffer: 0.2 mg of purified immunoglobulin in 0.5 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-Collagen IV antibody (ABIN135052)

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Friedrich, Bederson, Sehba: "Gender influences the initial impact of subarachnoid hemorrhage: an experimental investigation." in: PLoS ONE, Vol. 8, Issue 11, pp. e80101, (2015) (PubMed).

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Mao-Ying, Kavelaars, Krukowski, Huo, Zhou, Price, Cleeland, Heijnen: "The Anti-Diabetic Drug Metformin Protects against Chemotherapy-Induced Peripheral Neuropathy in a Mouse Model." in: PLoS ONE, Vol. 9, Issue 6, pp. e100701, (2014) (PubMed).

Wu, Davis, McMillen, Schaeffer, Zhou, Qi, Mazandarani, Alialy, Hudkins, Lusis, LeBoeuf: "Genetic modulation of diabetic nephropathy among mouse strains with Ins2 Akita mutation." in: Physiological reports, Vol. 2, Issue 11, (2014) (PubMed).

Abrahamson, St John, Stroganova, Zelenchuk, Steenhard: "Laminin and type IV collagen isoform substitutions occur in temporally and spatially distinct patterns in developing kidney glomerular basement membranes." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 61, Issue 10, pp. 706-18, (2013) (PubMed).

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Mirmalek-Sani, Orlando, McQuilling, Pareta, Mack, Salvatori, Farney, Stratta, Atala, Opara, Soker: "Porcine pancreas extracellular matrix as a platform for endocrine pancreas bioengineering." in: Biomaterials, Vol. 34, Issue 22, pp. 5488-95, (2013) (PubMed).

Manigrasso, Sawyer, Hutchens, Flynn, Maric-Bilkan: "Combined inhibition of aromatase activity and dihydrotestosterone supplementation attenuates renal injury in male streptozotocin (STZ)-induced diabetic rats." in: American journal of physiology. Renal physiology, (2012) (PubMed).

Manigrasso, Sawyer, Marbury, Flynn, Maric: "Inhibition of estradiol synthesis attenuates renal injury in male streptozotocin-induced diabetic rats." in: American journal of physiology. Renal physiology, Vol. 301, Issue 3, pp. F634-40, (2011) (PubMed).

Boyette-Davis, Cata, Zhang, Driver, Wendelschafer-Crabb, Kennedy, Dougherty: "Follow-up psychophysical studies in bortezomib-related chemoneuropathy patients." in: The journal of pain : official journal of the American Pain Society, Vol. 12, Issue 9, pp. 1017-24, (2011) (PubMed).

Liu, Chen, Huang, Chung, Zhou, Fu, Szalai, Lan: "C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes." in: Diabetologia, Vol. 54, Issue 10, pp. 2713-23, (2011) (PubMed).

Glorieux, Bachert, Favoreel, Vandekerckhove, Steukers, Rekecki, Van den Broeck, Goossens, Croubels, Clayton, Nauwynck: "Herpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa." in: PLoS ONE, Vol. 6, Issue 7, pp. e22160, (2011) (PubMed).

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Type IV Collagen (COL4 Products)

Synonyms: collagen, type IV antibody, Let-2 antibody