COL3 antibody

Catalog No. ABIN135050

Product Details anti-COL3 Antibody

Target: COL3 (Collagen, Type III (COL3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This COL3 antibody is un-conjugated

Application: ELISA

Specificity: Reacts with conformational determinants on type III collagen Referenced species reactivities include - Human 2,5,11,12,32,33,35-37 Chicken 22,23 Hamster 18,24 Mouse 4,6,13,15,34,39 Chinchilla 26 Porcine 1,5,17,29 Rat 5,10,14,20,31 Equine 25 Rabbit 9,16,19,21,38 Bovine 8,27 Feline 30 Rhesus 7 Canine 3,5 Guinea Pig 21 Tree Shrew 28

Cross-Reactivity (Details): Exhibits < 10 % cross reactivity with collagen type II, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

Characteristics: Goat Anti-Type III Collagen-UNLB

Purification: Purification Method: Affinity chromatography on type III collagen covalently linked to agarose.

Isotype: IgG

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA ,
• Other referenced applications include - IHC-PS , IHC-FS , ICC , EM , FC , WB
• Working Dilutions: ELISA BIOT conjugate 1:1,000 - 1:4,000

Sample Volume: 0,5 mL

Restrictions: For Research Use only

Handling

Concentration: 0.4 mg/mL

Buffer: 0.2 mg of purified immunoglobulin in 0.5 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-COL3 antibody (ABIN135050)

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Sembach, Fink, Johansen, Boland, Secher, Thrane, Nielsen, Fosgerau, Vrang, Jelsing, Pedersen, Østergaard: "Impact of sex on diabetic nephropathy and the renal transcriptome in UNx db/db C57BLKS mice." in: Physiological reports, Vol. 7, Issue 24, pp. e14333, (2020) (PubMed).

Becker, OConnell, Vo, Cain, Tampe, Bizarro, Sugimoto, McGow, Asara, Lovisa, McAndrews, Zielinski, Lorenzi, Zeisberg, Raza, LeBleu, Kalluri: "Epigenetic Reprogramming of Cancer-Associated Fibroblasts Deregulates Glucose Metabolism and Facilitates Progression of Breast Cancer." in: Cell reports, Vol. 31, Issue 9, pp. 107701, (2020) (PubMed).

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Dobie, Wilson-Kanamori, Henderson, Smith, Matchett, Portman, Wallenborg, Picelli, Zagorska, Pendem, Hudson, Wu, Budas, Breckenridge, Harrison, Mole, Wigmore, Ramachandran, Ponting, Teichmann, Marioni et al.: "Single-Cell Transcriptomics Uncovers Zonation of Function in the Mesenchyme during Liver Fibrosis. ..." in: Cell reports, Vol. 29, Issue 7, pp. 1832-1847.e8, (2020) (PubMed).

Welc, Flores, Wehling-Henricks, Ramos, Wang, Bertoni, Tidball: "Targeting a therapeutic LIF transgene to muscle via the immune system ameliorates muscular dystrophy." in: Nature communications, Vol. 10, Issue 1, pp. 2788, (2019) (PubMed).

Delestre-Delacour, Carmon, Laguerre, Estay-Ahumada, Courel, Elias, Jeandel, Rayo, Peinado, Sengmanivong, Gasman, Coudrier, Anouar, Montero-Hadjadje: "Myosin 1b and F-actin are involved in the control of secretory granule biogenesis." in: Scientific reports, Vol. 7, Issue 1, pp. 5172, (2019) (PubMed).

He, Zhang, Tolosa, Goru, Chen, Misra, Robinson, Yuen: "A new, easily generated mouse model of diabetic kidney fibrosis." in: Scientific reports, Vol. 9, Issue 1, pp. 12549, (2019) (PubMed).

Ellenbroek, de Haan, van Klarenbosch, Brans, van de Weg, Smeets, de Jong, Arslan, Timmers, Goumans, Hoefer, Doevendans, Pasterkamp, Meyaard, de Jager: "Leukocyte-Associated Immunoglobulin-like Receptor-1 is regulated in human myocardial infarction but its absence does not affect infarct size in mice." in: Scientific reports, Vol. 7, Issue 1, pp. 18039, (2019) (PubMed).

Qiao, Dai, Nikolova, Jin, Li, Xiao, Li, Moy, Xiao: "Amelioration of Muscle and Nerve Pathology in LAMA2 Muscular Dystrophy by AAV9-Mini-Agrin." in: Molecular therapy. Methods & clinical development, Vol. 9, pp. 47-56, (2018) (PubMed).

Wada, Kawada, Hirai, Toda, Iwamoto, Hasegawa, Sakai: "Enhanced anastomotic healing by Daikenchuto (TJ-100) in rats." in: Scientific reports, Vol. 8, Issue 1, pp. 1091, (2018) (PubMed).

Westendorp, Büller, Karpus, van Dop, Koster, Versteeg, Koelink, Snel, Meisner, Roelofs, Uhmann, Ver Loren van Themaat, Heijmans, Hahn, Muncan, Wildenberg, van den Brink: "Indian Hedgehog Suppresses a Stromal Cell-Driven Intestinal Immune Response." in: Cellular and molecular gastroenterology and hepatology, Vol. 5, Issue 1, pp. 67-82.e1, (2018) (PubMed).

de Bruyn, Becker, Steenkamer, Wildenberg, Meijer, Buskens, Bemelman, Löwenberg, Ponsioen, van den Brink, DHaens: "Intestinal fibrosis is associated with lack of response to Infliximab therapy in Crohn's disease." in: PLoS ONE, Vol. 13, Issue 1, pp. e0190999, (2018) (PubMed).

Pu, Zhang, Huang, Tian, He, Wang, Zhang, Liu, Li, Li, Zhao, Liu, Lu, Zhou, Huang, Nie, Yan, Hui, Lui, Zhou: "Mfsd2a+ hepatocytes repopulate the liver during injury and regeneration." in: Nature communications, Vol. 7, pp. 13369, (2018) (PubMed).

Poosti, Bansal, Yazdani, Prakash, Beljaars, van den Born, de Borst, van Goor, Hillebrands, Poelstra: "Interferon gamma peptidomimetic targeted to interstitial myofibroblasts attenuates renal fibrosis after unilateral ureteral obstruction in mice." in: Oncotarget, Vol. 7, Issue 34, pp. 54240-54252, (2018) (PubMed).

Hijmans, Shrestha, Sarpong, Yazdani, El Masri, de Jong, Navis, Vivès, van den Born: "High sodium diet converts renal proteoglycans into pro-inflammatory mediators in rats." in: PLoS ONE, Vol. 12, Issue 6, pp. e0178940, (2017) (PubMed).

Hijmans, Rasmussen, Yazdani, Navis, van Goor, Karsdal, Genovese, van den Born: "Urinary collagen degradation products as early markers of progressive renal fibrosis." in: Journal of translational medicine, Vol. 15, Issue 1, pp. 63, (2017) (PubMed).

Poosti, Pham, Oosterhuis, Poelstra, van Goor, Olinga, Hillebrands: "Precision-cut kidney slices (PCKS) to study development of renal fibrosis and efficacy of drug targeting ex vivo." in: Disease models & mechanisms, Vol. 8, Issue 10, pp. 1227-36, (2016) (PubMed).

Yazdani, Hijmans, Poosti, Dam, Navis, van Goor, van den Born: "Targeting tubulointerstitial remodeling in proteinuric nephropathy in rats." in: Disease models & mechanisms, Vol. 8, Issue 8, pp. 919-30, (2016) (PubMed).

Abdul, Dixon, Walker, Horabin, Smith, Weir, Brewster, Deehan, Mann, Borthwick: "Fibrosis is a common outcome following total knee arthroplasty." in: Scientific reports, Vol. 5, pp. 16469, (2016) (PubMed).

Target Details for COL3

Target: COL3 (Collagen, Type III (COL3))

Alternative Name: Type III Collagen (COL3 Products)

Synonyms: PT antibody, RPRGL2 antibody, THPH1 antibody, Cf-2 antibody, Cf2 antibody, FII antibody, thrombin antibody, prothrombin antibody, coagulation factor II, thrombin antibody, coagulation factor II antibody, F2 antibody