COL2 antibody

Catalog No. ABIN135048

Product Details anti-COL2 Antibody

Target: COL2 (Collagen, Type II (COL2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This COL2 antibody is un-conjugated

Application: ELISA

Specificity: Reacts with conformational determinants on type II collagen Referenced species reactivities include - Human 4,5,10,12,14,18-20,22,24,27,29,31,33,34 Chicken 16,26 Mouse 6,7,15 Porcine 21 Rat 13,17,25 Rabbit 2,9,11,30,32 Bovine 2,3,19,23 Sheep 8,28

Cross-Reactivity (Details): Exhibits < 10 % cross reactivity with collagen type II, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

Characteristics: Goat Anti-Type II Collagen-UNLB

Purification: Purification Method: Affinity chromatography on type II collagen covalently linked to agarose.

Isotype: IgG

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA
• Other referenced applications include - IHC-PS , IHC-FS , ICC , EM , WB , IP
• Working Dilutions: ELISA BIOT conjugate 1:1,000 - 1:4,000 FLISA AF488 conjugate 1:200 - 1:400

Sample Volume: 0,5 mL

Restrictions: For Research Use only

Handling

Concentration: 0.4 mg/mL

Buffer: 0.2 mg of purified immunoglobulin in 0.5 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-COL2 antibody (ABIN135048)

Tan Timur, Caron, van den Akker, van der Windt, Visser, van Rhijn, Weinans, Welting, Emans, Jahr: "Increased TGF-β and BMP Levels and Improved Chondrocyte-Specific Marker Expression In Vitro under Cartilage-Specific Physiological Osmolarity." in: International journal of molecular sciences, Vol. 20, Issue 4, (2019) (PubMed).

Kuwahara, Serowoky, Vakhshori, Tripuraneni, Hegde, Lieberman, Crump, Mariani: "Sox9+ messenger cells orchestrate large-scale skeletal regeneration in the mammalian rib." in: eLife, Vol. 8, (2019) (PubMed).

Fujii, Hirota, Yasoda, Takizawa, Morozumi, Nakamura, Yotsumoto, Kondo, Yamashita, Sakane, Kanai, Ueda, Yamauchi, Yamanaka, Nakao, Kuwahara, Jindo, Furuya, Mashimo, Inagaki, Serikawa, Nakao: "Rats deficient C-type natriuretic peptide suffer from impaired skeletal growth without early death." in: PLoS ONE, Vol. 13, Issue 3, pp. e0194812, (2018) (PubMed).

Caron, Emans, Sanen, Surtel, Cremers, Ophelders, van Rhijn, Welting: "The Role of Prostaglandins and COX-Enzymes in Chondrogenic Differentiation of ATDC5 Progenitor Cells." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153162, (2016) (PubMed).

Nakao, Osawa, Yasoda, Yamanaka, Fujii, Kondo, Koyama, Kanamoto, Miura, Kuwahara, Akiyama, Bessho, Nakao: "The Local CNP/GC-B system in growth plate is responsible for physiological endochondral bone growth." in: Scientific reports, Vol. 5, pp. 10554, (2016) (PubMed).

van den Akker, Surtel, Cremers, Rodrigues-Pinto, Richardson, Hoyland, van Rhijn, Welting, Voncken: "Novel immortal human cell lines reveal subpopulations in the nucleus pulposus." in: Arthritis research & therapy, Vol. 16, Issue 3, pp. R135, (2014) (PubMed).

Mirmalek-Sani, Orlando, McQuilling, Pareta, Mack, Salvatori, Farney, Stratta, Atala, Opara, Soker: "Porcine pancreas extracellular matrix as a platform for endocrine pancreas bioengineering." in: Biomaterials, Vol. 34, Issue 22, pp. 5488-95, (2013) (PubMed).

Target Details for COL2

Target: COL2 (Collagen, Type II (COL2))

Alternative Name: Type II Collagen (COL2 Products)