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Lambda-IgLC antibody (APC)

Lambda-IgLC Reactivity: Human FACS Host: Mouse Monoclonal 1-155-2 APC
Catalog No. ABIN1302655
  • Target See all Lambda-IgLC products
    Lambda-IgLC (lambda Light Chain (Lambda-IgLC))
    Reactivity
    • 25
    • 15
    Human
    Host
    • 22
    • 17
    • 1
    Mouse
    Clonality
    • 22
    • 18
    Monoclonal
    Conjugate
    • 10
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Lambda-IgLC antibody is conjugated to APC
    Application
    • 19
    • 14
    • 13
    • 13
    • 8
    • 7
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificity
    The mouse monoclonal antibody 1-155-2 recognizes lambda light chains (22.5 kDa) of human immunoglobulin.
    Cross-Reactivity (Details)
    Human
    Purification
    Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogen
    Human IgA1 lambda myeloma protein
    Clone
    1-155-2
    Isotype
    IgG1 kappa
  • Application Notes
    Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.
    Comment

    The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

    Restrictions
    For Research Use only
  • Reconstitution
    No reconstitution is necessary.
    Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Avoid prolonged exposure to light.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Tierens, Holte, Warsame, Ikonomou, Wang, Chan, Delabie: "Low levels of monoclonal small B cells in the bone marrow of patients with diffuse large B-cell lymphoma of activated B-cell type but not of germinal center B-cell type." in: Haematologica, Vol. 95, Issue 8, pp. 1334-41, (2010) (PubMed).

    Bahler, Pindzola, Swerdlow: "Splenic marginal zone lymphomas appear to originate from different B cell types." in: The American journal of pathology, Vol. 161, Issue 1, pp. 81-8, (2002) (PubMed).

    Kubagawa, Gathings, Levitt, Kearney, Cooper: "Immunoglobulin isotype expression of normal pre-B cells as determined by immunofluorescence." in: Journal of clinical immunology, Vol. 2, Issue 4, pp. 264-9, (1983) (PubMed).

  • Target
    Lambda-IgLC (lambda Light Chain (Lambda-IgLC))
    Alternative Name
    Lambda light chains (Lambda-IgLC Products)
    Background
    Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.,Immunoglobulin lambda, Igl
    Molecular Weight
    22.5 kDa
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