CD40 Ligand antibody

Catalog No. ABIN1302520

Product Details anti-CD40 Ligand Antibody

Target: CD40 Ligand (CD40LG)

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This CD40 Ligand antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Functional Studies (Func)

Specificity: The Armenian hamster monoclonal antibody MR-1 detects an extracellular epitope on murine CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.

Cross-Reactivity (Details): Mouse

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Activated mouse Th1 clone D1.6

Clone: MR-1

Isotype: IgG1

Application Details

Application Notes: Functional application:
Blocking T cell costimulation in vivo.
Flow cytometry: Recommended dilution: 4 μg/mL, positive control: 6-8 hour activated mouse splenocytes.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Preservative: Azide free

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD40 Ligand antibody (ABIN1302520)

Shin, Kennedy, Gorski, Tsuchiya, Koseki, Azuma, Yagita, Chen, Powell, Pardoll, Housseau: "Cooperative B7-1/2 (CD80/CD86) and B7-DC costimulation of CD4+ T cells independent of the PD-1 receptor." in: The Journal of experimental medicine, Vol. 198, Issue 1, pp. 31-8, (2003) (PubMed).

Luzina, Atamas, Storrer, daSilva, Kelsoe, Papadimitriou, Handwerger: "Spontaneous formation of germinal centers in autoimmune mice." in: Journal of leukocyte biology, Vol. 70, Issue 4, pp. 578-84, (2001) (PubMed).

Fischbein, Ardehali, Yun, Schoenberger, Laks, Irie, Dempsey, Cheng, Fishbein, Bonavida: "CD40 signaling replaces CD4+ lymphocytes and its blocking prevents chronic rejection of heart transplants." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 12, pp. 7316-22, (2000) (PubMed).

Hwang, Nahm, Briles, Thomas, Purkerson: "Acquired, but not innate, immune responses to Streptococcus pneumoniae are compromised by neutralization of CD40L." in: Infection and immunity, Vol. 68, Issue 2, pp. 511-7, (2000) (PubMed).

Lode, Xiang, Pertl, Förster, Schoenberger, Gillies, Reisfeld: "Melanoma immunotherapy by targeted IL-2 depends on CD4(+) T-cell help mediated by CD40/CD40L interaction." in: The Journal of clinical investigation, Vol. 105, Issue 11, pp. 1623-30, (2000) (PubMed).

Howard, Miga, Vanderlugt, Dal Canto, Laman, Noelle, Miller: "Mechanisms of immunotherapeutic intervention by anti-CD40L (CD154) antibody in an animal model of multiple sclerosis." in: The Journal of clinical investigation, Vol. 103, Issue 2, pp. 281-90, (1999) (PubMed).

Salgado, Nakamura, Sugaya, Tada, Asahina, Koyama, Irie, Tamaki: "Functional CD40 ligand is expressed on epidermal Langerhans cells." in: Journal of leukocyte biology, Vol. 66, Issue 2, pp. 281-5, (1999) (PubMed).

Noelle, Roy, Shepherd, Stamenkovic, Ledbetter, Aruffo: "A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 14, pp. 6550-4, (1992) (PubMed).

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD154 / CD40L (CD40LG Products)

Synonyms: CD154 antibody, cd40l antibody, CD40L antibody, HIGM1 antibody, IGM antibody, IMD3 antibody, T-BAM antibody, TNFSF5 antibody, TRAP antibody, gp39 antibody, hCD40L antibody, CD40-L antibody, Cd40l antibody, Ly-62 antibody, Ly62 antibody, Tnfsf5 antibody, CD40 ligand antibody, TNF superfamily member 5 antibody, CD40LG antibody, cd40l antibody, Cd40lg antibody

Background: CD40 ligand,CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.,CD40L, CD40 ligand, TNFSF5, Ly62, gp39, T-BAM

Gene ID: 959

UniProt: P29965

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints