SCIMP antibody

Catalog No. ABIN1302508

Product Details anti-SCIMP Antibody

Target: SCIMP (SLP Adaptor and CSK Interacting Membrane Protein (SCIMP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SCIMP antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC)

Specificity: The mouse monoclonal antibody NVL-07 recognizes intracellular part of human transmembrane adaptor SCIMP. This protein of 17 kDa predicted Mw migrates as a 22 kDa band on SDS PAGE.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Recombinant intracellular part of human SCIMP

Clone: NVL-07

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: Intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-SCIMP antibody (ABIN1302508)

Draber, Vonkova, Stepanek, Hrdinka, Kucova, Skopcova, Otahal, Angelisova, Horejsi, Yeung, Weiss, Brdicka: "SCIMP, a transmembrane adaptor protein involved in major histocompatibility complex class II signaling." in: Molecular and cellular biology, Vol. 31, Issue 22, pp. 4550-62, (2011) (PubMed).

Target Details for SCIMP

Target: SCIMP (SLP Adaptor and CSK Interacting Membrane Protein (SCIMP))

Alternative Name: SCIMP (SCIMP Products)

Synonyms: C17orf87 antibody, UNQ5783 antibody, A430084P05Rik antibody, SLP adaptor and CSK interacting membrane protein antibody, SCIMP antibody, Scimp antibody

Background: SLP adaptor and CSK interacting membrane protein,SCIMP (SLP adaptor and Csk interacting membrane protein), also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It is associated with tetraspanin-enriched microdomains (together with MHC II). There is a close relationship between SCIMP and tyrosinkinase Lyn, which is constitutively bound to it by its SH3 domain. After MHC II-mediated stimulation in the immunological synapse SCIMP becomes phosphorylated at several tyrosine residues and provides docking sites for Grb2 and SLP65 or SLP76 adaptors transducing the signal downstream, as well as for the kinase Csk with modulatory roles.,NVL

Gene ID: 388325

UniProt: Q6UWF3