IL3RA antibody

Catalog No. ABIN1302506

Product Details anti-IL3RA Antibody

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL3RA antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The mouse monoclonal antibody 6H6 recognizes an extracellular epitope of CD123 (interleukin 3 receptor alpha), a 60-70 kDa transmembrane protein expressed by myeloid precursors, megakaryocytes, macrophages, dendritic cells, mast cells, basophils, and some B cells. This antibody does not inhibit IL-3 binding to its receptor.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells

Clone: 6H6

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-IL3RA antibody (ABIN1302506)

Martín-Gayo, Sierra-Filardi, Corbí, Toribio: "Plasmacytoid dendritic cells resident in human thymus drive natural Treg cell development." in: Blood, Vol. 115, Issue 26, pp. 5366-75, (2010) (PubMed).

Herling, Teitell, Shen, Medeiros, Jones: "TCL1 expression in plasmacytoid dendritic cells (DC2s) and the related CD4+ CD56+ blastic tumors of skin." in: Blood, Vol. 101, Issue 12, pp. 5007-9, (2003) (PubMed).

Target Details for IL3RA

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Alternative Name: CD123 (IL3RA Products)

Synonyms: CD123 antibody, IL3R antibody, IL3RAY antibody, IL3RX antibody, IL3RY antibody, hIL-3Ra antibody, CDw123 antibody, SUT-1 antibody, Cyrl antibody, interleukin 3 receptor subunit alpha antibody, interleukin 3 receptor, alpha chain antibody, IL3RA antibody, Il3ra antibody

Background: Interleukin 3 receptor subunit alpha,CD123 is the alpha chain of interleukin 3 receptor (IL-3R alpha). This subunit heterodimerizes with the interleukin 3 receptor beta chain (CD131), which is shared with other receptors. CD123 interacts with IL-3 specifically, but with low affinity, and association with the beta subunit confers high affinity binding to the receptor heterodimer. Both chains are required for signaling, but receptor activation and signal transduction depend on IL-3 binding to CD123 as the initial step.,IL3RA, MGC34174, hIL-3Ra

Gene ID: 3563

UniProt: P26951

Pathways: JAK-STAT Signaling