Bcl-2 antibody (AA 41-54)

Catalog No. ABIN1302482

Product Details anti-Bcl-2 Antibody

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Binding Specificity: AA 41-54

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The mouse monoclonal antibody Bcl-2/100 recognizes Bcl2, a 26 kDa intracellular protooncogen with anti-apoptotic effect, expressed in outer mitochondrial membrane, endoplasmic reticulum and nuclear envelope.

No Cross-Reactivity: Mouse

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2

Clone: Bcl-2-100

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-Bcl-2 antibody (ABIN1302482)

Joubert, Marais, Maritz: "Influence of 2-methoxyestradiol on MCF-7 cells: an improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels." in: Biocell : official journal of the Sociedades Latinoamericanas de Microscopi?a Electronica ... et. al, Vol. 33, Issue 1, pp. 67-70, (2009) (PubMed).

Gugasyan, Christou, OReilly, Strasser, Gerondakis: "Bcl-2 transgene expression fails to prevent fatal hepatocyte apoptosis induced by endogenous TNFalpha in mice lacking RelA." in: Cell death and differentiation, Vol. 13, Issue 7, pp. 1235-7, (2006) (PubMed).

Laflamme, Israël-Assayag, Cormier: "Apoptosis of bronchoalveolar lavage lymphocytes in hypersensitivity pneumonitis." in: The European respiratory journal, Vol. 21, Issue 2, pp. 225-31, (2003) (PubMed).

Soilu-Hänninen, Ekert, Bucci, Syroid, Bartlett, Kilpatrick: "Nerve growth factor signaling through p75 induces apoptosis in Schwann cells via a Bcl-2-independent pathway." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 12, pp. 4828-38, (1999) (PubMed).

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: Bcl2 (BCL2 Products)

Synonyms: Bcl-2 antibody, PPP1R50 antibody, bcl-2 antibody, AW986256 antibody, C430015F12Rik antibody, D630044D05Rik antibody, D830018M01Rik antibody, BCL-2 antibody, Bcl2 antibody, BCL2, apoptosis regulator antibody, B cell leukemia/lymphoma 2 antibody, B-cell CLL/lymphoma 2a antibody, B-cell CLL/lymphoma 2 protein antibody, BCL2 antibody, Bcl2 antibody, bcl2a antibody, BCL-2 antibody

Background: BCL2 apoptosis regulator,Bcl2 (B cell chronic lymphatic leukemia protein 2) is a proto-oncogen, which can contribute to tumorigenesis by counteracting apoptosis in various cell types. The anti-apoptotic effect of Bcl2 is performed by its interactions with suppressors and agonists of cell death and under physiological conditions it is regulated by proteolytic processing and phosphorylation. Bcl2 expression can be detected mainly in lymphoid tissues and in the basal cells of epithelial tissues. It is also a marker that can help in classification of lymphoproliferative diseases and in prognostics of some epithelial neoplasms.,Bcl-2, PPP1R50

Gene ID: 596

UniProt: P10415

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling