EVI2B antibody

Catalog No. ABIN1302461

Product Details anti-EVI2B Antibody

Target: EVI2B (Ecotropic Viral Integration Site 2B (EVI2B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EVI2B antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificity: The mouse monoclonal antibody MEM-216 recognizes an extracellular epitope of CD361 / EVI2B, almost uncharacterized type I transmembrane protein with broad leukocyte expression, mostly in myeloid and B cells.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Raji cells

Clone: MEM-216

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL, Positive control, Positive control: Raji, Daudi, HL-60 cells, peripheral blood lymphcocytes (strongly positive on CD19+ cells), negative control: Jurkat, U-937 cells.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for EVI2B

Target: EVI2B (Ecotropic Viral Integration Site 2B (EVI2B))

Alternative Name: CD361 (EVI2B Products)

Synonyms: CD361 antibody, D17S376 antibody, EVDB antibody, Evi-2 antibody, Evi2 antibody, EVI2B antibody, ecotropic viral integration site 2B antibody, ecotropic viral integration site 2b antibody, EVI2B antibody, Evi2b antibody

Background: Ecotropic viral integration site 2B,CD361, also known as EVI2B (ecotropic viral integration site 2B) or EVDB, is a poorly characterized type I transmembrane protein, expressed from one of three genes embedded in intron 27b of the neurofibromatosis type 1 (NF1) gene. The DNA strand that is transcribed to produce CD361 is the complementary one to the strand encoding NF1. Murine homolog to human CD361 is associated with ecotropic viral insertions, which have been implicated in the expression of murine myeloid leukemias. CD361 has been also reported to be involved in melanocyte and keratinocyte differentiation. However, it is expressed mainly in peripheral blood and bone marrow.,EVI2B, EVDB

Gene ID: 2124

UniProt: P34910