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FOXP3 antibody

FOXP3 Reactivity: Human, Mouse WB, FACS Host: Mouse Monoclonal 3G3 unconjugated
Catalog No. ABIN1302424
  • Target See all FOXP3 Antibodies
    FOXP3 (Forkhead Box P3 (FOXP3))
    Reactivity
    • 152
    • 114
    • 46
    • 26
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 112
    • 57
    • 16
    • 2
    Mouse
    Clonality
    • 113
    • 74
    Monoclonal
    Conjugate
    • 85
    • 19
    • 13
    • 13
    • 11
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    This FOXP3 antibody is un-conjugated
    Application
    • 128
    • 79
    • 70
    • 37
    • 37
    • 27
    • 27
    • 18
    • 16
    • 16
    • 9
    • 8
    • 8
    • 7
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS)
    Specificity
    The mouse monoclonal antibody 3G3 recognizes N-terminal region of FoxP3, a 47-55 kDa transcription factor (intracellular antigen), which is the master regulator in the development and function of regulatory T cells.
    Cross-Reactivity (Details)
    Human, Mouse
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Full-length His-tagged recombinant murine FoxP3
    Clone
    3G3
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product FOXP3 Primary Antibody
  • Application Notes
    Western blotting: Recommended dilution: 2 μg/mL.
    Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Law, Hirschkorn, Owen, Biswas, Norris, Lanteri: "The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 75, Issue 12, pp. 1040-50, (2009) (PubMed).

    Gavin, Torgerson, Houston, DeRoos, Ho, Stray-Pedersen, Ocheltree, Greenberg, Ochs, Rudensky: "Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 17, pp. 6659-64, (2006) (PubMed).

  • Target
    FOXP3 (Forkhead Box P3 (FOXP3))
    Alternative Name
    FoxP3 (FOXP3 Products)
    Synonyms
    foxp3 antibody, FOXP3 antibody, AIID antibody, DIETER antibody, IPEX antibody, PIDX antibody, XPID antibody, JM2 antibody, scurfin antibody, sf antibody, RGD1562112 antibody, forkhead box P3 antibody, forkhead box P3a antibody, forkhead box P3 L homeolog antibody, FOXP3 antibody, foxp3a antibody, foxp3 antibody, foxp3.L antibody, Foxp3 antibody
    Background
    Forkhead box P3,FoxP3 (forkhead box protein 3), a highly conserved forkhead/winged-helix transcription factor, plays a crucial role in maintaining immune homeostasis by governing the development and function of regulatory T cells. It is constitutively expressed at high level in CD25+ CD4+ Treg cells and at low level in a CD25- CD4+ Treg cell subset. Defects in gene encoding FoxP3 protein cause the scurfy phenotype in mice, and in human the IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome.,Scurfin, JM2, AIID, IPEX, PIDX, XPID, DIETER
    Gene ID
    50943
    UniProt
    Q9BZS1
    Pathways
    Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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