PSMA antibody (AA 44-750)

Catalog No. ABIN1302364

Product Details anti-PSMA Antibody

Target: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Binding Specificity: AA 44-750

Reactivity: Human, Mouse, Rat, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This PSMA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Specificity: The mouse monoclonal antibody GCP-04 recognizes amino acids 100-104 of extracellular domain of denaturated glutamate carboxypeptidase II (PSMA, NAALADase, FOLH1), an approximately 95-110 kDa transmembrane glycoprotein.

Cross-Reactivity (Details): Human, Porcine, Mouse, Rat, Other not determined

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Recombinant fragment of human GCPII (amino acids 44-750) produced in S2 cells

Clone: GCP-04

Isotype: IgG1

Application Details

Application Notes: Western blotting: Recommended dilution: 1 μg/mL, positive control: LNCaP cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mL cold lysis buffer (1 % NP-40). Incubate 30 min on ice. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Both reducing and non-reducing conditions.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-PSMA antibody (ABIN1302364)

Wächter, Di Fazio, Maurer, Manoharan, Keber, Pfestroff, Librizzi, Bartsch, Luster, Eilsberger: "Prostate-Specific Membrane Antigen in Anaplastic and Poorly Differentiated Thyroid Cancer-A New Diagnostic and Therapeutic Target?" in: Cancers, Vol. 13, Issue 22, (2021) (PubMed).

Schreiber, Hänze, Nimphius, Verburg, Luster, Hofmann, Hegele: "Prostate specific membrane antigen (PSMA) in urothelial cell carcinoma (UCC) is associated with tumor grading and staging." in: Journal of cancer research and clinical oncology, (2020) (PubMed).

Gao, Xu, Cui, Zhang, Lin, Cai, Wang, Luo, Zheng, Wang, Luo, Jiang, Neale, Zhong: "Mice lacking glutamate carboxypeptidase II develop normally, but are less susceptible to traumatic brain injury." in: Journal of neurochemistry, Vol. 134, Issue 2, pp. 340-53, (2015) (PubMed).

Tykvart, Navrátil, Sedlák, Corey, Colombatti, Fracasso, Koukolík, Ba?inka, Sácha, Konvalinka: "Comparative analysis of monoclonal antibodies against prostate-specific membrane antigen (PSMA)." in: The Prostate, Vol. 74, Issue 16, pp. 1674-90, (2014) (PubMed).

Rovenská, Hlouchová, Sácha, Mlcochová, Horák, Zámecník, Barinka, Konvalinka: "Tissue expression and enzymologic characterization of human prostate specific membrane antigen and its rat and pig orthologs." in: The Prostate, Vol. 68, Issue 2, pp. 171-82, (2007) (PubMed).

Sácha, Zámecník, Barinka, Hlouchová, Vícha, Mlcochová, Hilgert, Eckschlager, Konvalinka: "Expression of glutamate carboxypeptidase II in human brain." in: Neuroscience, Vol. 144, Issue 4, pp. 1361-72, (2007) (PubMed).

Barinka, Mlcochová, Sácha, Hilgert, Majer, Slusher, Horejsí, Konvalinka: "Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding." in: European journal of biochemistry / FEBS, Vol. 271, Issue 13, pp. 2782-90, (2004) (PubMed).

Barinka, Sácha, Sklenár, Man, Bezouska, Slusher, Konvalinka: "Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity." in: Protein science : a publication of the Protein Society, Vol. 13, Issue 6, pp. 1627-35, (2004) (PubMed).

Barinka, Rinnová, Sácha, Rojas, Majer, Slusher, Konvalinka: "Substrate specificity, inhibition and enzymological analysis of recombinant human glutamate carboxypeptidase II." in: Journal of neurochemistry, Vol. 80, Issue 3, pp. 477-87, (2002) (PubMed).

Target Details for PSMA

Target: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Alternative Name: GCPII / PSMA (FOLH1 Products)

Synonyms: FGCP antibody, FOLH antibody, GCP2 antibody, GCPII antibody, NAALAD1 antibody, NAALAdase antibody, PSM antibody, PSMA antibody, mGCP antibody, mopsm antibody, Naalad antibody, putative N-acetylated-alpha-linked acidic dipeptidase antibody, folate hydrolase 1B antibody, folate hydrolase 1 antibody, folate hydrolase (prostate-specific membrane antigen) 1 antibody, LOC451185 antibody, FOLH1B antibody, FOLH1 antibody, Folh1 antibody

Background: Folate hydrolase 1,Glutamate carboxypeptidase II (GCPII), also known as N-acetyl-alpha-linked acidic dipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membrane antigen (PSMA), is an approximately 95-110 kDa type II transmembrane glycoprotein expressed in various tissues. In nervous system GCPII cleaves abundant N-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner, to N-acetylaspartate and glutamate. As immoderate glutamate concentration is neurotoxic, GCPII contributes to pathological conditions regarding e.g. Alzheimer´s disease, Huntington´s disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interesting therapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-gamma-glutamate to folate and glutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII, which is present in a number of tissues at low levels, is overexpressed in neovasculature of most solid tumours and is a target enzyme for diagnosis and treatment of prostate cancer. Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated at the N-terminus (PSM´) than mRNA for membrane-bound GCPII, and this ratio is reversed upon malignant transformation.,GCP2, FOLH1, NAALADase I, PGGCP, FGGCP, FGCP,

Gene ID: 2346

UniProt: Q04609