MEK1/2 antibody

Catalog No. ABIN1302344

Product Details anti-MEK1/2 Antibody

Target: MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK1/2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: The polyclonal antibody reacts with intracellular antigens MEK 1/2. MEK 1 and MEK 2 are integral components of the MAP kinase cascade, an important pathway for cell growth and differentiation.

Purification: Purified by ligand affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Synthetic peptide (coupled with KLH) derived from aminoacid sequence 33-53 of human MAPK Kinase 1. The immunogen sequence is highly conserved within species (human, mouse, rat, hamster, bovine etc.).

Isotype: IgG

Application Details

Application Notes: Western blotting: Recommended dilution 0.5 μg/mL, positive material: RAJI human Burkitt lymphoma cell line, both reducing and non-reducing conditions, reducing conditions are recommended.

Comment:

Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human), Phosphotyrosine)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for MEK1/2

Target: MEK1/2 (MAP2K1/2) (Mitogen-Activated Protein Kinase Kinase 1/2 (MAP2K1/2))

Alternative Name: MAP2K1/2 (MAP2K1/2 Products)

Background: MEK (MAPKK, kinase of mitogen-activated protein kinase) is the medium one of three components of MAP kinase cascade –, an important signaling pathway that regulates cell growth and differentiation. Raf (MAPKKK) activates MEK 1 and 2 via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK 1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK (MAPK, mitogen-activated protein kinase). This phosphorylation of ERK by MEK 1/2 is a critical step in the MAP kinase cascade. Phosphorylated ERK is capable of translocating to the nucleus, where it regulates gene expression by activating transcription factors.,MAPKK 1/2, MKK1/2