MHC Class II antibody

Catalog No. ABIN1301917

Product Details anti-MHC Class II Antibody

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This MHC Class II antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Specificity: The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.

Cross-Reactivity (Details): Mouse

Purification: Purified by protein-G affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Activated C57BL/6 mouse spleen cells

Clone: M5-114

Isotype: IgG2b

Application Details

Application Notes: Functional application: Blocking of T cell proliferative responses.
Flow cytometry: Recommended dilution: 2 μg/mL.
Immunohistochemistry: Recommended dilution: 5-10 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Preservative: Azide free

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-MHC Class II antibody (ABIN1301917)

Kuwano, Prazma, Yazawa, Watanabe, Ishiura, Kumanogoh, Okochi, Tamaki, Fujimoto, Tedder: "CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells." in: International immunology, Vol. 19, Issue 8, pp. 977-92, (2007) (PubMed).

Beers, Burich, Kleijmeer, Griffith, Wong, Rudensky: "Cathepsin S controls MHC class II-mediated antigen presentation by epithelial cells in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 3, pp. 1205-12, (2005) (PubMed).

Zang, Kalache, Lin, Schroppel, Murphy: "MHC Class II-mediated apoptosis by a nonpolymorphic MHC Class II peptide proceeds by activation of protein kinase C." in: Journal of the American Society of Nephrology : JASN, Vol. 16, Issue 12, pp. 3661-8, (2005) (PubMed).

Kleijmeer, Ramm, Schuurhuis, Griffith, Rescigno, Ricciardi-Castagnoli, Rudensky, Ossendorp, Melief, Stoorvogel, Geuze: "Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells." in: The Journal of cell biology, Vol. 155, Issue 1, pp. 53-63, (2001) (PubMed).

Clausen, Waldburger, Schwenk, Barras, Mach, Rajewsky, Förster, Reith: "Residual MHC class II expression on mature dendritic cells and activated B cells in RFX5-deficient mice." in: Immunity, Vol. 8, Issue 2, pp. 143-55, (1998) (PubMed).

De Souza Leao, Lang, Prina, Hellio, Antoine: "Intracellular Leishmania amazonensis amastigotes internalize and degrade MHC class II molecules of their host cells." in: Journal of cell science, Vol. 108 ( Pt 10), pp. 3219-31, (1995) (PubMed).

Viville, Neefjes, Lotteau, Dierich, Lemeur, Ploegh, Benoist, Mathis: "Mice lacking the MHC class II-associated invariant chain." in: Cell, Vol. 72, Issue 4, pp. 635-48, (1993) (PubMed).

Bhattacharya, Dorf, Springer: "A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2488-95, (1982) (PubMed).

Target Details for MHC Class II

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Alternative Name: MHC Class II (MHC2 Products)

Synonyms: H-2 antibody, MHC-II antibody, histocompatibility-2, MHC antibody, H2 antibody

Background: MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.