GFP antibody (Biotin)

Catalog No. ABIN129729

Supplier Product No.: 600-406-215

Product Details anti-GFP Antibody (Biotin)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFP antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: 600-406-215

Supplier: Rockland

Purpose: GFP Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria).

Characteristics: Synonyms: rabbit anti-GFP antibody biotin conjugation, biotin conjugated rabbit anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Purification: Anti-GFP was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Biotin Conjugated GFP Antibody has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:10,000 - 1:50,000

IF Microscopy Dilution: 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Anti-GFP at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-GFP antibody (Biotin) (ABIN129729)

Lee, Taylor, Barnes, Shen, Stewart, Chen, Xiang, Bao, Shen: "A Myt1 family transcription factor defines neuronal fate by repressing non-neuronal genes." in: eLife, Vol. 8, (2020) (PubMed).

Wen, Gao, Wang, Ray, Magnuson, Wright, Di Magliano, Frankel, Crawford: "Myeloid Cell-Derived HB-EGF Drives Tissue Recovery After Pancreatitis." in: Cellular and molecular gastroenterology and hepatology, Vol. 8, Issue 2, pp. 173-192, (2019) (PubMed).

Wang, Vukovic, Koh, Schulten, Myong: "Dynamic profiling of double-stranded RNA binding proteins." in: Nucleic acids research, Vol. 43, Issue 15, pp. 7566-76, (2015) (PubMed).

Lu, Tremblay, King, Qi, Reynolds, Marker, Varrone, Majewska, Dewhurst, Gelbard: "HIV-1 Tat-induced microgliosis and synaptic damage via interactions between peripheral and central myeloid cells." in: PLoS ONE, Vol. 6, Issue 9, pp. e23915, (2011) (PubMed).

Zangar, Daly, White, Servoss, Tan, Collett: "ProMAT calibrator: A tool for reducing experimental bias in antibody microarrays." in: Journal of proteome research, Vol. 8, Issue 8, pp. 3937-43, (2009) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Background: Background: GFP Antibody is produced from Green fluorescent protein which is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.

UniProt: P42212