GFP antibody (AA 246) (Biotin)

Catalog No. ABIN129729

Product Details anti-GFP Antibody (Biotin)

Target: GFP (Green Fluorescent Protein (GFP))

Binding Specificity: AA 246

Reactivity: Aequorea victoria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFP antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunomicroscopy (IM)

Cross-Reactivity: recombinant GFP (rGFP), enhanced GFP (eGFP)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence derived from the jellyfish Aequorea victoria.
Immunogentype:Recombinant

Isotype: IgG

Application Details

Application Notes: Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-GFP antibody (Biotin) (ABIN129729)

Lee, Taylor, Barnes, Shen, Stewart, Chen, Xiang, Bao, Shen: "A Myt1 family transcription factor defines neuronal fate by repressing non-neuronal genes." in: eLife, Vol. 8, (2020) (PubMed).

Wen, Gao, Wang, Ray, Magnuson, Wright, Di Magliano, Frankel, Crawford: "Myeloid Cell-Derived HB-EGF Drives Tissue Recovery After Pancreatitis." in: Cellular and molecular gastroenterology and hepatology, Vol. 8, Issue 2, pp. 173-192, (2019) (PubMed).

Wang, Vukovic, Koh, Schulten, Myong: "Dynamic profiling of double-stranded RNA binding proteins." in: Nucleic acids research, Vol. 43, Issue 15, pp. 7566-76, (2015) (PubMed).

Lu, Tremblay, King, Qi, Reynolds, Marker, Varrone, Majewska, Dewhurst, Gelbard: "HIV-1 Tat-induced microgliosis and synaptic damage via interactions between peripheral and central myeloid cells." in: PLoS ONE, Vol. 6, Issue 9, pp. e23915, (2011) (PubMed).

Zangar, Daly, White, Servoss, Tan, Collett: "ProMAT calibrator: A tool for reducing experimental bias in antibody microarrays." in: Journal of proteome research, Vol. 8, Issue 8, pp. 3937-43, (2009) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Synonyms: green fluorescent protein antibody, gfp antibody

Background: Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.