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RFP antibody (HRP)

Rockland 600-403-379 RFP Reactivity: Discosoma WB, ELISA, IHC Host: Rabbit Polyclonal HRP
Rockland
Catalog No. ABIN129725
Supplier Product No.: 600-403-379
  • Target See all RFP Antibodies
    RFP (Red Fluorescent Protein (RFP))
    Reactivity
    Discosoma
    Host
    • 21
    • 13
    • 9
    • 5
    • 3
    Rabbit
    Clonality
    • 30
    • 21
    Polyclonal
    Conjugate
    • 18
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This RFP antibody is conjugated to HRP
    Application
    • 25
    • 19
    • 14
    • 11
    • 11
    • 8
    • 7
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Supplier Product No.
    600-403-379
    Supplier
    Rockland
    Purpose
    RFP Antibody Peroxidase Conjugated Pre-Adsorbed
    Cross-Reactivity (Details)
    Minimal crossreactivity against Hu Ms & Rt Serum Proteins
    Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.
    Characteristics
    Synonyms: rabbit anti-RFP antibody peroxidase conjugation, rabbit anti-Red Fluorescent Protein antibody peroxidase conjugation, HRP conjugated rabbit anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
    Purification

    Rabbit Anti-RFP Peroxidase Conjugated Antibody was prepared from monospecific rabbit antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    Preadsorption: Pre-adsorbed

    Immunogen

    Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

    Immunogen Type: Recombinant Protein

    Isotype
    IgG
    Top Product
    Discover our top product RFP Primary Antibody
  • Application Notes

    Immunohistochemistry Dilution: 1:500 - 1:2,000

    Application Note: Rabbit Anti-RFP Peroxidase Conjugated Antibody has been tested in ELISA and Western blot. It can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen or to detect RFP or RFP containing proteins on Western blots. References suggest that the antibody is also suitable for IHC. Optimal titers for applications should be determined by the researcher.

    Western Blot Dilution: 1:2,000 - 1:5,000

    ELISA Dilution: 1:10,000 - 1:50,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Bohlen, El-Nahal, Sommer: "Transduction of Craniofacial Motoneurons Following Intramuscular Injections of Canine Adenovirus Type-2 (CAV-2) in Rhesus Macaques." in: Frontiers in neuroanatomy, Vol. 13, pp. 84, (2019) (PubMed).

  • Target
    RFP (Red Fluorescent Protein (RFP))
    Alternative Name
    DsRed (RFP Products)
    Background
    Background: Fluorescent proteins such as Discosoma Red Fluorescent Protein (DsRed) from sea anemone Discosoma sp. mushroom or green fluorescent protein (GFP) from Aequorea victoria jellyfish are widely used in research practice. Fusion RFP and GFP commonly serve as marker for gene expression and protein localization. As DsRed and GFP share only 19 % identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional step in the chromophore maturation and obligates tetrameric structure. Rockland offers many controls, monoclonal, and polyclonal antibodies for RFP.
    UniProt
    Q9U6Y8
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